Question about assemble long-read sequencing data with short-read polishing about rna-bloom HOT 3 OPEN

I see that you started with 7.6 million reads in stage 3. You must have a lot of long reads as input!

Except their corresponding short reads under 25 degree, I have the short reads under 32 degree for the animals. I am wondering should I use all the short reads for polishing?

Yes, you should provide all short reads for polishing.

Actually, I have tried both options. But I have some problem in understanding the output log file. Could you please tell me what does G: |V| mean? also, |E|? What is 'dovetail reads'? Thank you very much!

|V| and |E| are the number of vertices and edges in the graph.

"Dovetail reads" are those that have dovetail overlaps, e.g.

Read 1:  ============
Overlap:       ||||||
Read 2:        ==============

from rna-bloom.

Thank you for your explanation! I have a further question, for the assembled file, how could I know which transcript is the isform with which transcripts?

from rna-bloom.

RNA-Bloom does not report that information.

from rna-bloom.