Image merge stitch about pyvips HOT 2 OPEN

Hello @alexlsa,

I've not looked at your images, but the general advice is:

• Camera focal plane: the sensor need to be co-planar with both your object, and with the plane of movement of the translation stage. This can be very hard to get exactly right :( The last time I did this I spent a long time driving the stage left/right/up/down and seeing how specks of dust on the object moved. Each time, you remove the camera, insert shims, and try again.

• Lens distortion: pincushioning or barrelling needs to be less then a few pixels. There are various ways of measuring and correcting this, the simplest is to get a grating target and image that.

• Translation stage errors: these are never exactly straight, and especially errors in the cutting of the screws can be significant. It depends how good your stage is. Again, it'd be worth trying to characterise your stage.

A simple experiment is to drive the stage to 100 positions, chosen to get a speck of dust positioned in a 10 x 10 grid filling the field of view of the camera. Find the pixel (x, y) position of the point in each frame and see if those points have a linear relationship, ie. are square-ish. If they are not, you have some uncorrected errors somewhere you need to fix.

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Thank you @jcupitt ,

Sorry I'm late, I will check and try your recommendations to get a good result

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