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yeban avatar yeban commented on August 31, 2024

Check the contents of run/h99_longest_transcript/lifted_cleanup.logmaybe

from flo.

yeban avatar yeban commented on August 31, 2024

(and let me know too)

from flo.

cmatKhan avatar cmatKhan commented on August 31, 2024

I'm reasonably sure at this point that this is a problem with the gff I am using, specifically the feature IDs in the 3rd column. If I can come up with a suggestion for some error handling, I'll go ahead and post it. Otherwise, this is probably a idiosyncratic problem on my side rather than something to handle in the software itself.

from flo.

Ist4lri avatar Ist4lri commented on August 31, 2024

Hi; I have the same problem than @cmatKhan just show in his picture.
I would like to know how we can fix the problems. This is last lines of the run/Longest_Transcript/lifted_cleanup.log :

Chr1	kh2012	three_prime_UTR	5073729	5073960	.	+	.	Parent=KH2012:KH.C1.8.v5.A.ND1-1;Target=KH.C1.8.v5.A.ND1-1 900 1131
Chr1	kh2012	three_prime_UTR	2551018	2551052	.	+	.	Parent=KH2012:KH.C1.5.v2.A.ND1-1;Target=KH.C1.5.v2.A.ND1-1 4516 4550
Chr1	kh2012	three_prime_UTR	2551316	2551581	.	+	.	Parent=KH2012:KH.C1.5.v2.A.ND1-1;Target=KH.C1.5.v2.A.ND1-1 4551 4807
Chr1	kh2012	three_prime_UTR	5670475	5670962	.	+	.	Parent=KH2012:KH.C1.1056.v1.A.ND1-1;Target=KH.C1.1056.v1.A.ND1-1 1631 2118
warning: line 1 in file "-" does not begin with "##gff-version" or "##gvf-version", create "##gff-version 3" line automatically
gt gff3: error: Parent "KH2012:KH.C1.976" on line 1 in file "-" was not defined (via "ID=")

Any idea or hint for how to correct this ?

I'm using the gff file attached with this message.

I trying to watch RakeFile and gff_recover.rb but didn't see any problem, or hint for solutions...
Longest_Transcript.txt

from flo.

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