Giter VIP home page Giter VIP logo

Comments (2)

LTLA avatar LTLA commented on August 11, 2024

exprs() itself is not deprecated, but now points to the "logcounts" assay, as this was the typical use.

In this specific case, we still store the log-transformed "exprs" in the output, but exprs() doesn't point there anymore. To get at it, you'll need to do assay(sce, "exprs") or the equivalent.

Admittedly, this is all a bit confusing, so in the next release, I will remove the "exprs" output completely from readKallistoResults, i.e., only counts and TPMs are reported. If users want log-transformed TPMs, they can do it themselves and store the results in a "log_tpm" assay or something.

Personally, I don't think it makes much sense to add an offset of 1 when log-transforming CPM, TPM or FPKM values, see my rant here: https://support.bioconductor.org/p/104943/#104963

from scater.

bruvellu avatar bruvellu commented on August 11, 2024

Ah, I see now. Thanks for the detailed explanations!

from scater.

Related Issues (20)

Recommend Projects

  • React photo React

    A declarative, efficient, and flexible JavaScript library for building user interfaces.

  • Vue.js photo Vue.js

    🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.

  • Typescript photo Typescript

    TypeScript is a superset of JavaScript that compiles to clean JavaScript output.

  • TensorFlow photo TensorFlow

    An Open Source Machine Learning Framework for Everyone

  • Django photo Django

    The Web framework for perfectionists with deadlines.

  • D3 photo D3

    Bring data to life with SVG, Canvas and HTML. 📊📈🎉

Recommend Topics

  • javascript

    JavaScript (JS) is a lightweight interpreted programming language with first-class functions.

  • web

    Some thing interesting about web. New door for the world.

  • server

    A server is a program made to process requests and deliver data to clients.

  • Machine learning

    Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.

  • Game

    Some thing interesting about game, make everyone happy.

Recommend Org

  • Facebook photo Facebook

    We are working to build community through open source technology. NB: members must have two-factor auth.

  • Microsoft photo Microsoft

    Open source projects and samples from Microsoft.

  • Google photo Google

    Google ❤️ Open Source for everyone.

  • D3 photo D3

    Data-Driven Documents codes.