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DeadlineWasYesterday avatar DeadlineWasYesterday commented on July 28, 2024

Hmm. I think I have traced the source of the problem. I ran multiple (too many) instances of bowtie using different combinations of flags to see if it was any of the flags that was causing the problem. Then I was wondering whether the memory buffer on the HPC was generating the same results in spite of me changing the files (The m7b.fa file did previously have duplicate headers). So I switched to a different computer node and ran bowtie again, but the problem was still there.

Then I tried changing the computer node and building the index again, and that apparently solved the problem.

TL;DR: I believe it was the memory buffer. The program is fine.

from bowtie.

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