Comments (3)
I'm also not sure why you need to use STAR to align first and unmapped reads were aligned again with bwa.
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Because STAR tolerates reads with a mismatch at the spliced junction sites (i.e., linear mapping)
BUT has a low tolerate rate for reads with a mismatch at the chimeric junction sites (i.e., invert mapping). For this part of reads, I used bwa to further detect chimeric junctions.
For Supplementary Alignment,
from ricpipe.
Thank you !
In my result, there is a cluster that the left arm overlaps the right arm.
"chr12 8053387 8053492 chr12 8053466 8053492 FianlCluster_57"
Does this mean there is something wrong with my program?
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Related Issues (8)
- What is the rRNA sequence, genome reference HOT 11
- issues while calling intermolecular interaction HOT 2
- count_link_for_each_kind.pl cannot get gapped reads number HOT 1
- STAR should be "2.5.x", not "v020201" HOT 1
- Speed of STAR aligner is extremely low HOT 3
- How to prepare the gene annotation files HOT 7
- issues when running the step5.screen_high-confidence_intermolecular HOT 2
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