Giter VIP home page Giter VIP logo

Comments (3)

ZHIDIHUAYUAN avatar ZHIDIHUAYUAN commented on August 13, 2024

I'm also not sure why you need to use STAR to align first and unmapped reads were aligned again with bwa.

from ricpipe.

caochch avatar caochch commented on August 13, 2024

Because STAR tolerates reads with a mismatch at the spliced junction sites (i.e., linear mapping)
BUT has a low tolerate rate for reads with a mismatch at the chimeric junction sites (i.e., invert mapping). For this part of reads, I used bwa to further detect chimeric junctions.

For Supplementary Alignment,

from ricpipe.

ZHIDIHUAYUAN avatar ZHIDIHUAYUAN commented on August 13, 2024

Thank you !
In my result, there is a cluster that the left arm overlaps the right arm.
"chr12 8053387 8053492 chr12 8053466 8053492 FianlCluster_57"
Does this mean there is something wrong with my program?

from ricpipe.

Related Issues (8)

Recommend Projects

  • React photo React

    A declarative, efficient, and flexible JavaScript library for building user interfaces.

  • Vue.js photo Vue.js

    🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.

  • Typescript photo Typescript

    TypeScript is a superset of JavaScript that compiles to clean JavaScript output.

  • TensorFlow photo TensorFlow

    An Open Source Machine Learning Framework for Everyone

  • Django photo Django

    The Web framework for perfectionists with deadlines.

  • D3 photo D3

    Bring data to life with SVG, Canvas and HTML. 📊📈🎉

Recommend Topics

  • javascript

    JavaScript (JS) is a lightweight interpreted programming language with first-class functions.

  • web

    Some thing interesting about web. New door for the world.

  • server

    A server is a program made to process requests and deliver data to clients.

  • Machine learning

    Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.

  • Game

    Some thing interesting about game, make everyone happy.

Recommend Org

  • Facebook photo Facebook

    We are working to build community through open source technology. NB: members must have two-factor auth.

  • Microsoft photo Microsoft

    Open source projects and samples from Microsoft.

  • Google photo Google

    Google ❤️ Open Source for everyone.

  • D3 photo D3

    Data-Driven Documents codes.