Comments (2)
Hi Assa,
The problem is that you've not provided the input files via the appropriate -1
and -2
flags. For your particular command, the command line should look like
$ salmon quant -p 16 --biasCorrect --libType IU -1 <(zcat ${base}_1.fastq.gz ) -2 <(zcat ${base}_2.fastq.gz) -i ~./Salmon/Salmon.index/Homo_sapiens.GRCh38.rel79/ --numBootstraps 100 -o $base
Notice this looks almost the same as what you had before, except that the reads are prefixed by the relevant -1
and -2
flags, and come directly after the library type flags. Please let me know if this fixes the issue for you. I'll add a more informative message in such a situation.
from salmon.
thanks. Now it seems to work. I close this issue.
from salmon.
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from salmon.