Comments (5)
Best solution is to just mask the version of diamond distributed with anaconda and supply your own. I don't know who is updating the anaconda distribution, but I might recommend updating the version of diamond supplied with it.
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Hello? Can you tell me in detail how to solve the problem when Diamand cannot be used? Thank you vary much!
from orthofinder.
Hello? Can you tell me in detail how to solve the problem when Diamand cannot be used? Thank you vary much!
I downloaded OrthoFinder over anaconda, so it was located in a folder with a name like ~/envs/orthofinder/bin
. There should also be a program in that folder named diamond
. Rename this file to something like diamond_DEPRECATED
or delete it altogether. Then go to the diamond github page (https://github.com/bbuchfink/diamond) and download the file for your system. Then unpack the file and retrieve the diamond executable. Either put it in ~/envs/orthofinder/bin
or some other place where it will be found in your PATH
variable.
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I did this and succeeded, but there was a warning, as follows, have you ever encountered it? Thank you!
WARNING: program called by OrthoFinder produced output to stderr
Command: diamond blastp --ignore-warnings -d /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/diamondDBSpecies27 -q /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/Species36.fa -o /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/Blast36_27.txt --more-sensitive -p 1 --quiet -e 0.001 --compress 1
stdout
b''
stderr
b'Warning: Failed to delete temporary file /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/diamond-tmp-DioQLv\n'
from orthofinder.
I did this and succeeded, but there was a warning, as follows, have you ever encountered it? Thank you!
WARNING: program called by OrthoFinder produced output to stderr
Command: diamond blastp --ignore-warnings -d /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/diamondDBSpecies27 -q /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/Species36.fa -o /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/Blast36_27.txt --more-sensitive -p 1 --quiet -e 0.001 --compress 1
stdout
b''
stderr
b'Warning: Failed to delete temporary file /mnt/share/2024.1.22/11/tri40/OrthoFinder/Results_Jan22_1/WorkingDirectory/diamond-tmp-DioQLv\n'
I have not ever had that error but I don't think it's a problem. You can always delete temporary files manually if you need to.
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Related Issues (20)
- [Bug:] Option `-p tmp_pickle_dir` not working
- [Feature request] reduce number generated files per directory (e.g. for blast results, pickle files)
- ERROR when starting from BLAST, Orthogroups or Tree
- Rooted species tree branch length meaning HOT 1
- Is it possible to detect Orthologous Isoforms
- Only two species comparison, but the number of one-to-one orthologs in Orthologues Stats one_to_one.tsv is more than the number of single-copy orthogroups in statistics Overall.tsv file
- DockerFile
- OSError: [Errno 24] Too many open files [32514] Failed to execute script 'orthofinder' due to unhandled exception!
- Orthogroups vs Superfamily Family Tree
- Get order of concatenated sequences in SpeciesTreeAlignment.fa
- is it possible to specify a tree for orthofinder? HOT 1
- Identifying number of characters involved in species tree inference using -M msa option
- ValueError: setting an array element with a sequence.
- ERROR: external program called by OrthoFinder returned an error code: -9
- Inferring gene trees with for orthogroups with less than 4 sequences HOT 1
- Which directories for versions of Orthofinder v2.4.0 (or more recent) are valid with the HOGs?
- Issue with rerunning orthofinder "from trees" step -- Blast0_0.txt not found HOT 1
- -T raxml-ng——ERROR: Species tree inference failed HOT 1
- Duplicates of the same gene in Orthogroup sequence files HOT 3
- "Invalid escape sequence" errors while starting OrthoFinder HOT 1
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