Giter VIP home page Giter VIP logo

Comments (3)

fmalmeida avatar fmalmeida commented on August 15, 2024

One possibility could be the following structure:

{outdir}
├── final_output
│   └── illumina / nanopore / pacbio                       # one directory for each sequencing technology
│       │   └──  all reads in ".fq.gz" format
├── preprocessing_outputs
│   └── illumina / nanopore / pacbio                       # one directory for each sequencing technology
│       │   └──   QC, trimming, filtering, correction, demultiplexing etc. (whenever available)

# files of all samples will be placed inside the QC, trimming, filtering, etc directories. Each file will have the sample ID to make selection easier.

What do you think about such structure, @gpappasunb?

from ngs-preprocess.

fmalmeida avatar fmalmeida commented on August 15, 2024

I will also take advantage of the changes, to also change the software used for illumina preprocessing ---

Instead of using 4 different tools (fastqc, trim_galore, lighter and flash) I will use only fastp which has all the functionalities of these 4 tools and is much faster since it is written in C++

from ngs-preprocess.

fmalmeida avatar fmalmeida commented on August 15, 2024

The change to fastp has already been addressed. The documentation needs to be properly changed prior to making a new release (v2.4).

Now, with fastp and following the new structure of outputs, it would look like this:

TESTE
├── final_output
│   ├── illumina
│   │   ├── SRR8482585_30X.merged.fq.gz
│   │   ├── SRR8482585_30X_R1.unmerged.fq.gz
│   │   └── SRR8482585_30X_R2.unmerged.fq.gz
│   ├── nanopore
│   │   └── kp_30X.filtered.fq.gz
│   └── pacbio
│       └── lima.bc1018--bc1018.filtered.fq.gz
└── preprocessing_outputs
    ├── illumina
    │   ├── SRR8482585_30X_fastp.html
    │   └── SRR8482585_30X_fastp.json
    ├── nanopore
    │   ├── QC
    │   │   ├── kp_30X_nanoQC
    │   │   ├── kp_30X_nanoplot
    │   │   └── kp_30X_stats
    │   └── porechop
    │       └── kp_30X.trimmed.fq.gz
    └── pacbio
        ├── QC
        │   ├── lima.bc1018--bc1018_nanoQC
        │   ├── lima.bc1018--bc1018_nanoplot
        │   └── lima.bc1018--bc1018_stats
        └── bam2fastq
            ├── lima.bc1018--bc1018.bam.pbi
            └── lima.bc1018--bc1018.fq.gz

All these changes are already available in branch develop and are being tracked by the draft PR #9.

from ngs-preprocess.

Related Issues (13)

Recommend Projects

  • React photo React

    A declarative, efficient, and flexible JavaScript library for building user interfaces.

  • Vue.js photo Vue.js

    🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.

  • Typescript photo Typescript

    TypeScript is a superset of JavaScript that compiles to clean JavaScript output.

  • TensorFlow photo TensorFlow

    An Open Source Machine Learning Framework for Everyone

  • Django photo Django

    The Web framework for perfectionists with deadlines.

  • D3 photo D3

    Bring data to life with SVG, Canvas and HTML. 📊📈🎉

Recommend Topics

  • javascript

    JavaScript (JS) is a lightweight interpreted programming language with first-class functions.

  • web

    Some thing interesting about web. New door for the world.

  • server

    A server is a program made to process requests and deliver data to clients.

  • Machine learning

    Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.

  • Game

    Some thing interesting about game, make everyone happy.

Recommend Org

  • Facebook photo Facebook

    We are working to build community through open source technology. NB: members must have two-factor auth.

  • Microsoft photo Microsoft

    Open source projects and samples from Microsoft.

  • Google photo Google

    Google ❤️ Open Source for everyone.

  • D3 photo D3

    Data-Driven Documents codes.