Comments (3)
Yes, that should still be possible, but you may need to do the annotations of all the genomes included if the resources are scant. And Paramecium is one of the species with a PantherHMM database. You can have a look at the list for the most closely related species you may want to use.
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Thank you for getting back to me so quickly. I am currently working with green Tetrahymena, which we have sequenced and annotated. My plan is to compare it with all of the other reported full genome sequences of Tetrahymena species from the TGD database. I am wondering if it is necessary to use the pather_HMM database for this pipeline. My ultimate goal is to study gene expansion/contraction and analyze Ka/Ks.
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No worries. That's also possible if you want to classify the orthogroups (output of OrthoFinder) yourself. You may work on the output of OrthoFinder directly if you wish and perform CAFE expansion/contraction analyses on them with or without classifying the orthogroups (i.e. attaching biological function or most likely gene families to each orthogroup).
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Related Issues (20)
- Make use of Nextflow's IO more effectively
- Improve documentation and debug Venn diagram plotting HOT 3
- Rerunning from orthogroups appends species names to protein sequence names which conflicts with downstream module expectations of HOT 1
- Process `PLOT` terminated with an error exit status (1) HOT 39
- Allow for PANTHER databases to be linked instead of downloaded
- HyPhy integration for more efficient detection of signatures of selection on gene sequences
- Performance assessment on polyploid genomes
- Error executing process > 'PLOT' HOT 32
- Error on test dataset HOT 4
- Diagnosing pipeline failure HOT 11
- cannot open file 'ORTHOGROUPS_SINGLE_GENE.NT.timetree.nex': No such file or directory HOT 17
- assess_specific_genes is taking days HOT 6
- Can't generate venn diagram with R script HOT 5
- RE: 4DTv and WGD HOT 5
- cannot open file 'CAFE_results/Gamma_clade_results.txt': No such file or directory HOT 2
- assess_specific_genes.nf fails to open ORTHOGROUPS_SINGLE_GENE.NT.treefile HOT 2
- How to visualize GO term enrichment analysis output? HOT 2
- Extracting species specific gene families expansion and contraction
- grep: GSTU13_CAFE_results/Gamma_clade_results.txt: No such file or directory
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