Comments (3)
This issue happened after v1.6.0 when "blastn" is not executable. You can prove this by executing /home/svillanu/Programs/GetOrganelle/GetOrganelleDep/linux/ncbi-blast/blastn -version
directly, you would probably get "/lib64/libc.so.6: version `GLIBC_2.14' not found". This happened because the attached ncbi-blast version may be too high for your system. I guess you got this issue because you updated GetOrganelle after you finished the previous successful chloroplast running.
One temporary solution for this is ①if you installed GetOrganelle in the in situ
way, delete the folder /home/svillanu/Programs/GetOrganelle/GetOrganelleDep/linux/ncbi-blast/
(GetOrganelle would then automatically use the blastn
in the $PATH); ②if you installed GetOrganelle in the setup.py
way, delete the folder /home/svillanu/Programs/GetOrganelle/GetOrganelleDep/linux/ncbi-blast/
then use the pip
to reinstall. I guess you used the in situ
way.
I would fix or at least give better instruction for this issue in the coming v1.6.2 (under testing and almost done). Good luck with your study!
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You were absolutely right, GLIBC_2.14 was the problem. I have been familiar with this kind of issues with other programs.
I deleted the folder as you said, and installed blast from bioconda in the same environment where I put the python packages. Seems like it solved the problem as both are running right now.
Just as a curiosity, have you considered making a bioconda package for GetOrganelle? Would make it way easier to install and I think would help to alleviate this kind of dependencies issues.
Thanks for the fast reply and the solution!
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Thanks for the suggestion. I would add it to the plan, although it would not be very soon.
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