Comments (3)
The input of "summary_get_organelle_output.py" is the output folder(s), (after the flag "-o" in your "get_organelle_from_reads.py" command). e.g. you have multiple samples finished with
get_organelle_from_reads.py -1 sample1_1.fq.gz -2 sample1_2.fq.gz -t 1 -o sample1-plastome -F embplant_pt -R 10
get_organelle_from_reads.py -1 sample2_1.fq.gz -2 sample2_2.fq.gz -t 1 -o sample2-plastome -F embplant_pt -R 10
get_organelle_from_reads.py -1 sample3_1.fq.gz -2 sample3_2.fq.gz -t 1 -o sample3-plastome -F embplant_pt -R 10
Then, you can summarize those results with
summary_get_organelle_output.py sample_1-plastome sample_2-plastome sample_3-plastome -o plastome_run_stat.tab
or
summary_get_organelle_output.py sample*-plastome -o plastome_run_stat.tab
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Thanks for posting this! I just realize that I forgot to update the description/termination for "evaluate_assembly_using_mapping.py". It's outdated and inaccurate. Now I have correct the description in the code.
It SHOULD BE "Matched: 375.33±66.39 (375.33±66.39)", NOT "Aligned: 375.33±66.39 (375.33±66.39)", meaning the mean and standard deviation of matched bases per site (depth/coverage) of the mapping alignment.
The format can be described as
Matched: global_depth_mean±global_depth_stdev (contig_depth_mean±contig_depth_stdev)
Refer to Reads mapping to evaluate plastome assemblies in the manuscript (https://www.biorxiv.org/content/10.1101/256479v4.full). The biorxiv version was actually updated again with minor clarification in our submission version to the journal, but the idea is the same.
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Thank you very much. I can successfully run the script:summary_get_organelle_output.py. Thank you for your explanation of the Matched: 375.33±66.39 (375.33±66.39).
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Related Issues (20)
- ERROR: Assembling failed due to SPAdes failure HOT 4
- More sequence.fasta?
- strand + or - ? HOT 1
- Issue : Invalid characters # Whitespace not allowed HOT 2
- Using embplant_mt and embplant_pt not possible HOT 2
- GetOrganelle aborts with error ("Killed") HOT 3
- Problems with canu-produced gfa processing HOT 1
- Program killed during early extension; RAM issue unlikely HOT 2
- ERROR: slimming the pre-assembled graph failed
- AssertionError: Invalid characters (e.g. space, non-ascii) for SPAdes in path: /home/ruizhang/zhaoliaocheng/Viburnum cpDNA/LYL47/plastome_output
- 2023-05-24 14:15:33,428 - INFO: Round 9: 10081574/10081574 AI 89119 AW 2714392 2023-05-24 14:15:33,428 - INFO: No more reads found and terminated ... 2023-05-24 14:16:02,153 - INFO: Extending finished. HOT 1
- incomplete assembly graph HOT 2
- Discrepancy between get_organelle_from_reads and summary_get_organelle_output logs HOT 1
- Set Startingpoint of Cicular Mitochondrial Genome HOT 2
- conda install error: conda version 23.5.0 too high HOT 2
- Please explain the output - multiple fasta HOT 1
- assembly_graph.fastg finished with no target organelle contigs found! HOT 7
- empty filtered paired HOT 2
- Problems with chloroplast genome assembly stagnation HOT 4
- Invalid information provided HOT 1
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