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macmanes avatar macmanes commented on July 26, 2024

Can you confirm for me that you have edited the config.ini file as per https://oyster-river-protocol.readthedocs.io/en/latest/aws_setup.html#edit-config-ini?

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jpwares avatar jpwares commented on July 26, 2024

At first I went OY! because I had done so the 1st time I tried to install ORP but seem to have missed the second. However I fixed that this morning, tried again no luck, tried again with a clean sample data directory, failed in same way.

WARNING The dataset you provided does not contain the file dataset.cfg, likely because it is an old version. Default species (fly, eukaryota) will be used as augustus species
ERROR Impossible to read $HOME/Oyster_River_Protocol/busco_dbs/eukaryota_odb9/
mv: cannot stat ‘run_test*’: No such file or directory
make: *** [/home/jpwares/Oyster_River_Protocol/sampleclone2/reports/test.busco.done] Error 1

BELOW my /software/config.ini file:
[busco]
lineage_path = $HOME/Oyster_River_Protocol/busco_dbs/eukaryota_odb9
force = True
quiet = False

[tblastn]
path = $HOME/Oyster_River_Protocol/software/anaconda/install/envs/orp_v2/bin/

[makeblastdb]
path = $HOME/Oyster_River_Protocol/software/anaconda/install/envs/orp_v2/bin/

[hmmsearch]
path = $HOME/Oyster_River_Protocol/software/anaconda/install/envs/orp_v2/bin/

thanks
John

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macmanes avatar macmanes commented on July 26, 2024

ok, few things to change and check..

in config.ini, change the $HOME/Oyster.. to /home/jpwares/Oyster... At one point BUSCO could not read variables.

Can you check to see that $HOME/Oyster_River_Protocol/busco_dbs/eukaryota_odb9 versus $HOME/Oyster_River_Protocol/sampleclone2/busco_dbs/eukaryota_odb9 actually exists?

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jpwares avatar jpwares commented on July 26, 2024

aw hell you caught me being lazy with my path. fixed. thank you very much, sorry for the novice error.

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