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katrinr avatar katrinr commented on August 18, 2024

Figured it out, maybe useful for others, so I post it here

[DEFAULT]                                                                                                                               
psiblast = <path_to_packages>/packages/databases/psi-blast/ncbi-blast-2.8.1+                                                                
uniref90 = <path_to_packages>/packages/databases/uniref90/uniref90.fasta                                                                    
hhblits = <path_to_packages>/packages/hh-suite/build/bin/hhblits                                                                            
uniprot20 = <path_to_packages>/packages/databases/uniprot20_2016_02/uniprot20_2016_02 

Btw thanks for the tool!

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mircare avatar mircare commented on August 18, 2024

HHblits is a bit confusing in this respect so I've just improved the description of uniprot20.

Thank you for using Porter5!

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DRL36 avatar DRL36 commented on August 18, 2024

I encountered the same issue here and I've tried changing the default, but it still went wrong. For me, 2FLGA.fasta.flatpsi is created, but no 2FLGA.flatpsi.ann file. Any ideas on how to fix this?
btw, I commented out psiblast and uniref90 since I'm running the fast prediction and those 2 are not needed.

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mircare avatar mircare commented on August 18, 2024

@DRL36 Please try the below command and open another issue with more details if the problem persists.

python3 Porter5.py -i example/2FLGA.fasta --cpu 4 --setup

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