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maxulysse avatar maxulysse commented on September 24, 2024

Hi @grendon, I'm not sure this is due to the singularity container.
These WARNINGS in the .command.err seems concerning to me:

WARNING	2023-04-18 15:20:56	CollectAlignmentSummaryMetrics	No Read length histograms to plot.
WARNING	2023-04-18 15:20:56	CollectBaseDistributionByCycle	No valid bases found in input file. No plot will be produced.
WARNING	2023-04-18 15:20:56	CollectInsertSizeMetrics	All data categories were discarded because they contained < 0.05 of the total aligned paired data.
WARNING	2023-04-18 15:20:56	CollectInsertSizeMetrics	Total mapped pairs in all categories: 0.0
WARNING	2023-04-18 15:20:56	MeanQualityByCycle	No valid bases found in input file. No plot will be produced.
WARNING	2023-04-18 15:20:56	QualityScoreDistribution	No valid bases found in input file. No plot will be produced.
[Tue Apr 18 15:20:56 GMT 2023] picard.analysis.CollectMultipleMetrics done. Elapsed time: 0.00 minutes.

Can you check if the files are not empty, and if not, that singularity is properly configured so that Nextflow can read the files properly?

from viralrecon.

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