Comments (3)
I think I should filter out the sequences perhaps both before blast and after blast. The ECR11 sequence doesn't give many blast hits outside of primates, so perhaps removing the LINE from that prior to blast will improve the hits in other species.
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Okay, I've now (in commit 6418a80) removed repeats from the data pipeline both prior to blasting (I blast with both the masked and unmasked sequence to get better hits) and after. However, I'm going to run into an issue when I get to the final selection calling, since that assumes that the coordinates in the MPRA data match the human sequence, which they no longer necessarily do.
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Alright, with the latest bolus of commits, I think I have everything fixed. I ran into an additional issue where different regions were getting masked, so I got around that by propagating the masks after a final alignment.
The coordinate issue I solved by reinserting a homo sapiens sequence with a different name, and having all my scripts know not to mask that.
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Related Issues (11)
- Decide what to do when there's not complete saturation of the mutagenesis HOT 1
- Merge Reconstructions doesn't work with snakemake on the cluster
- Think more closely about what to do with bases where we have data on deletions
- Make sure to process exonic enhancers differently HOT 2
- Deal with internal node if sequence same as parent
- Make changes to allow software to run more easily on different computers HOT 1
- BLAST can sometimes not return any hits in Rodents HOT 1
- Think about comparison with ancestral reconstruction MPRAs HOT 1
- Have some method to specify target ingroup HOT 1
- Come up with a figure or three on biological results HOT 2
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