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benjjneb avatar benjjneb commented on July 19, 2024

Is there currently a QIIME object for paired fastq files (or lists of paired fastq files)?

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johnchase avatar johnchase commented on July 19, 2024

I don't believe that there is. @ebolyen can you confirm?

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ebolyen avatar ebolyen commented on July 19, 2024

There is if I understand the question correctly: SampleData[PairedEndSequencesWithQuality] which uses SingleLanePerSamplePairedEndFastqDirFmt as its backing directory format.

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benjjneb avatar benjjneb commented on July 19, 2024

If that exists already, then adding a paired end workflow should be straightforward.

Is there documentation of SampleData[PairedEndSequencesWithQuality] objects? For example, can it be assumed there are always forward and reverse files for each sample?

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ebolyen avatar ebolyen commented on July 19, 2024

No real documentation, and we can't yet enforce that there exists a forward and reverse for each sample, but I believe it is our goal to be able to validate that property. That particular directory format does contain a manifest file which maps the sample id to the filepath and direction.

relevant lines for format
manifest definition
q2-demux which manipulates these

These parts of the API (multi-file directory formats) are still very rough around the edges, but we're thinking about ways to make it easier.

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benjjneb avatar benjjneb commented on July 19, 2024

Added in a1857e5 and b66432d

Assumes dada2 1.2+

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