Comments (4)
Hi
My guess is that you have a cas operon in "cas_operons_putative.tab" on Contig51 with no definite subtype, which is close to a CRISPR array. Maybe it's cas1 and cas2, which seems missing from the I-B cas operon on Contig137. I can see that the output is confusing in this case
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These are the results for contig 51 on cas_operons_putative.tab
file:
Contig Operon Start End Prediction Complete_Interference Complete_Adaptation Best_type Best_score Genes Positions E-values CoverageSeq CoverageHMM Strand_Interference Strand_Adaptation
Contig51 Contig51@1 785 2608 Ambiguous ['0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%', '0%'] ['100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', '100%', 'NA'] ['I-A', 'I-B', 'I-C', 'I-D', 'I-G', 'II-B', 'V-A', 'V-B1', 'V-B2', 'V-E', 'V-F1', 'V-F2', 'V-F3', 'V-F'] 6.0 ['Cas4_5_CAS-I-II-III-IV-V-VI', 'Cas1_6_CAS-I-II-III-IV-V-VI', 'Cas2_2_CAS-I-II-III-IV-V-VI'] [2, 3, 4] ['1.30e-49', '1.90e-120', '8.40e-24'] [0.976, 0.964, 0.789] [1.0, 0.994, 0.975] NA 1
It seems a bit ambiguous, but I show the results in case the same thing happens to another user
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Yeah, so you have an adaptation module (cas1, cas2, cas4) next to a CRISPR array on contig51, and the best guess cctyper can give is I-B due to the CRISPR repeat sequence. And then you have a I-B interference module on contig137
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Thanks!
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Related Issues (20)
- FileNotFoundError: blast.tab HOT 3
- Complete genome and draft genome HOT 2
- A complement to CRISRPRCasFinder or its own thing? HOT 1
- Execution against protein fasta and gff HOT 2
- Different results from CRISPR Cas typer web server and standalone version HOT 5
- Error when running cctyper: sed: cannot rename <output filename>/sed78a7GM: Permission denied HOT 2
- ![image](https://user-images.githubusercontent.com/121949837/212599561-d771b233-4bde-4850-8e5f-98a4164f78cb.png)
- Option to use Prodigal-gv
- Meaning of asterisk at the end of protein sequence HOT 1
- Creating empty files when there's no data HOT 1
- castyping.py having an issue with positional arguments. HOT 2
- Orphan CRISPR definition HOT 2
- (Question) provide GFF file for pre computed gene calls? HOT 5
- XGBoost model incompatible HOT 8
- If minced detects a CRISPR with N's in the repeat sequence
- (Question) How can I plot svg post-hoc after a run? HOT 1
- ValueError: invalid literal for int() with base 10: 'lengt' HOT 2
- access to sequences that used to geenrate Cas profiles
- access to sequences that used to geenrate Cas profiles HOT 1
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