Comments (3)
skovaka
commented on July 17, 2024
I believe this sometimes happens when you try to run UNCALLED before you start the sequencing run. Try starting the sequencing run first, wait until the target temperature is reached, then run UNCALLED. As long you start before the first mux scan ends you will not miss any reads. Sorry about that, I know the README suggests you run UNCALLED before starting a run. I will try to a solution to this bug or else update the README, but that should work in the meantime.
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ChloeDG
commented on July 17, 2024
Ohhhhhhh, I see. Yup looks like it is working well after I start a run! Great, thank you!
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ChloeDG
commented on July 17, 2024
FYI working well for me! I see a nice enrichment when I exclude 'short' reads.
:-)
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Related Issues (20)
- [QUERY] Can I test UNCALLED to always try and map only 2000 raw samples from FAST5 HOT 1
- Uncalled in ubuntu 16 HOT 3
- Segmentation fault in 'uncalled sim' HOT 3
- Floating point exception HOT 8
- uncalled failed to connect to minknow instance HOT 3
- Running Uncalled using Flongle flow cell HOT 2
- Updated Uncalled and Minknow No Longer Working HOT 4
- Generating sequencing summary from fast5 raw reads HOT 1
- [QUESTION] mapping to reference stringency HOT 1
- Installing UNCALLED4 error HOT 12
- [QUESTION] 10X run-time for reads of 500 raw signals HOT 12
- Visualising f5c resquiggle output in UNCALLED4 HOT 6
- Computer requirement for UNCALLED HOT 1
- `sim` segmentation error HOT 7
- Installation on Ubuntu (issue with compiler) HOT 5
- Sequenced reads are too short HOT 4
- New release HOT 2
- Remove mux scan windows from Flongle run HOT 1
- Error when trying example HOT 12
- Fast5 file `vbz` problem HOT 2
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