Error when running uncalled map about uncalled HOT 5 CLOSED

Try installing the VBZ compression plugin: https://github.com/nanoporetech/vbz_compression
ONT recently changed their compression format, which didn't cause any problems when I tested it, but it looks like you might need to install it manually. Let me know if that works and I'll working on automating that, or at least add a note to the readme

from uncalled.

Thanks for the quick response!

Unfortunately this didn't work for me. I am still getting the same error. I'm thinking that maybe it has to do with my file, however I'm not sure what's the hang-up.

Would you mind quickly testing or looking at this file? It is from a multi-read-fast5 file where I split it into single read files. The multi read file was downloaded from the pass directory saved by the Mk1C nanopore.

Thanks

https://pivotbio-my.sharepoint.com/:u:/p/ncolaianni/EfXpVVzJBq9BqHl9a0GQcXoB1Xf1ICBeUVybiGDYT1P4kQ?e=PeFWJC

from uncalled.

Thanks for sharing! Looks like it's the same issue reported here: nanoporetech/vbz_compression#5
If you're on Linux (and maybe Mac too) try running export HDF5_PLUGIN_PATH=/usr/local/hdf5/lib/plugin, then run uncalled map.
"/usr/local/hdf5/lib/plugin" should contain a file called "libvbz_hdf_plugin.so". If that command doesn't work and you don't see that file, you'll need to figure out where the VBZ installer put the plugin library. Hope that helps!

from uncalled.

Ah, the last piece! It worked like a charm. Thanks a lot!

from uncalled.

No problem, glad it worked!

from uncalled.