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saketkc avatar saketkc commented on August 19, 2024

It automatically determines the most informative read lengths to use (based on if the metagene distribution for a given read length exceeds the cutoff): https://github.com/smithlabcode/ribotricer/blob/master/ribotricer/detect_orfs.py#L360. The profiles are then merged by determine an appropriate offset that maximizes the cross-correlation.

You can override this behavior by providing a list of read lengths to use and their corresponding p-site offsets:

"--read_lengths",

"Comma separated P-site offsets for each read length "

Feel free to reopen if you have any other questions.

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lbwfff avatar lbwfff commented on August 19, 2024

It automatically determines the most informative read lengths to use (based on if the metagene distribution for a given read length exceeds the cutoff): https://github.com/smithlabcode/ribotricer/blob/master/ribotricer/detect_orfs.py#L360. The profiles are then merged by determine an appropriate offset that maximizes the cross-correlation.

You can override this behavior by providing a list of read lengths to use and their corresponding p-site offsets:

"--read_lengths",

"Comma separated P-site offsets for each read length "

Feel free to reopen if you have any other questions.

Hi, saketkc

I understand that Ribotricer will Assessing the Periodicity of Read Length, but will Ribotricer assess all the read length in the BAM file? Even if it is far greater than or less than 30bp.

Thanks, leelee

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saketkc avatar saketkc commented on August 19, 2024

Yes

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