Comments (3)
It could be due to incompatible data format. Have you tried to prepare the data as in the manual?
from dbg2olc.
@yechengxi yes, I followed the Step 0 in the manual to generate PacBio_CorrectTrimmed_30x.fasta
Step 0.1: Prepare PacBio data from error-corrected, trimmed PacBio reads. This step completed succesfully
/DBG2OLC/compiled/SelectLongestReads sum 18000000000 longest 1 o PacBio_CorrectTrimmed_30x.fasta f SampleID.trimmedReads.fasta.gz
Step 0.2: Prepare Illumina data.
/DBG2OLC/compiled/SelectLongestReads sum 24000000000 longest 0 o Illumina_PE_40x.fasta f PE_1.fastq.gz f PE_2.fastq.gz
Although the Illumina reads are all with the same length (100bp), this step FAILED with
terminate called after throwing an instance of 'std::length_error'
what(): basic_string::resize
~/.lsbatch/1476834650.83211: line 8: 28505 Aborted (core dumped)
Step 1. De novo assembly of Illumina data (with ALLPATHS-lg). The contigs.fasta file was created
Step 2. Run DBG2OLC using input created in Step 1 and Step 0.1
/DBG2OLC/DBG2OLC k 17 AdaptiveTh 0.001 KmerCovTh 2 MinOverlap 15 RemoveChimera 1 Contigs $contigs f PacBio_CorrectTrimmed_30x.fasta
This failed with exceptions that I reported in my first post
*** Error in `/software/bioinformatics/DBG2OLC/DBG2OLC': free(): invalid pointer: 0x0000000022ddec42 ***
Can you spot anything wrong in the above steps? How can we fix the error and move on? Thank you.
from dbg2olc.
NOte that in step 0.2 you need to unzip the gz files first.
from dbg2olc.
Related Issues (20)
- Empty sequence loaded. It looks like you have messed up the data. HOT 3
- settings for 10x and 7x coverage
- segmentation fault HOT 5
- Linked reads + PacBio reads HOT 1
- Empty sequence loaded. It looks like you have messed up the data. HOT 1
- No release management? HOT 2
- Just give you some suggestions! HOT 1
- Floating point exception (core dumped) HOT 1
- merging of final backbone contigs to illumina assembly HOT 1
- Overlap and Layout Step HOT 1
- segfault HOT 1
- Smaller consensus. HOT 1
- How to use paired-end reads (R1 and R2) in SparseAssembler HOT 3
- 0 reads loaded -> floating point exception HOT 3
- Sparc always uses 64 CPU threads
- Error! Maximal alignment length reached. max_len: 250 HOT 2
- FreeSparseKmerGraph 出现coredump
- Is it ContigTh or ContigCovTh? HOT 1
- can dbg2olc restarts at where it fails
- Empty sequence loaded. It looks like you have messed up the data. Assembly finished. HOT 1
Recommend Projects
-
React
A declarative, efficient, and flexible JavaScript library for building user interfaces.
-
Vue.js
🖖 Vue.js is a progressive, incrementally-adoptable JavaScript framework for building UI on the web.
-
Typescript
TypeScript is a superset of JavaScript that compiles to clean JavaScript output.
-
TensorFlow
An Open Source Machine Learning Framework for Everyone
-
Django
The Web framework for perfectionists with deadlines.
-
Laravel
A PHP framework for web artisans
-
D3
Bring data to life with SVG, Canvas and HTML. 📊📈🎉
-
Recommend Topics
-
javascript
JavaScript (JS) is a lightweight interpreted programming language with first-class functions.
-
web
Some thing interesting about web. New door for the world.
-
server
A server is a program made to process requests and deliver data to clients.
-
Machine learning
Machine learning is a way of modeling and interpreting data that allows a piece of software to respond intelligently.
-
Visualization
Some thing interesting about visualization, use data art
-
Game
Some thing interesting about game, make everyone happy.
Recommend Org
-
Facebook
We are working to build community through open source technology. NB: members must have two-factor auth.
-
Microsoft
Open source projects and samples from Microsoft.
-
Google
Google ❤️ Open Source for everyone.
-
Alibaba
Alibaba Open Source for everyone
-
D3
Data-Driven Documents codes.
-
Tencent
China tencent open source team.
from dbg2olc.