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yechengxi avatar yechengxi commented on June 11, 2024

It could be due to incompatible data format. Have you tried to prepare the data as in the manual?

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CeciliaDeng avatar CeciliaDeng commented on June 11, 2024

@yechengxi yes, I followed the Step 0 in the manual to generate PacBio_CorrectTrimmed_30x.fasta

Step 0.1: Prepare PacBio data from error-corrected, trimmed PacBio reads. This step completed succesfully
/DBG2OLC/compiled/SelectLongestReads sum 18000000000 longest 1 o PacBio_CorrectTrimmed_30x.fasta f SampleID.trimmedReads.fasta.gz

Step 0.2: Prepare Illumina data.
/DBG2OLC/compiled/SelectLongestReads sum 24000000000 longest 0 o Illumina_PE_40x.fasta f PE_1.fastq.gz f PE_2.fastq.gz

Although the Illumina reads are all with the same length (100bp), this step FAILED with

terminate called after throwing an instance of 'std::length_error'
what(): basic_string::resize
~/.lsbatch/1476834650.83211: line 8: 28505 Aborted (core dumped)

Step 1. De novo assembly of Illumina data (with ALLPATHS-lg). The contigs.fasta file was created

Step 2. Run DBG2OLC using input created in Step 1 and Step 0.1
/DBG2OLC/DBG2OLC k 17 AdaptiveTh 0.001 KmerCovTh 2 MinOverlap 15 RemoveChimera 1 Contigs $contigs f PacBio_CorrectTrimmed_30x.fasta

This failed with exceptions that I reported in my first post

*** Error in `/software/bioinformatics/DBG2OLC/DBG2OLC': free(): invalid pointer: 0x0000000022ddec42 ***

Can you spot anything wrong in the above steps? How can we fix the error and move on? Thank you.

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yechengxi avatar yechengxi commented on June 11, 2024

NOte that in step 0.2 you need to unzip the gz files first.

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