Comments (5)
With this command the fasta files and the ReadsInfoFrom_*.fasta files should be located in the working directory. It looks like the fasta files are not though?
from dbg2olc.
You mean the (raw) PacBio fasta files? Does it need those?
from dbg2olc.
from dbg2olc.
I have clarified this in the updated manual. Thanks for letting me know.
from dbg2olc.
Hi! I got the same error message when trying to use two long reads files, one from PacBio and another from MinION. Does DBG2OLC support both kinds of files?
Here's what I did:
nohup /DATOS/pgaiero/DBG2OLC_git/compiled/DBG2OLC k 17 AdaptiveTh 0.001 KmerCovTh 2 MinOverlap 20 RemoveChimera 1 Contigs /DATOS/pgaiero/Paspalum_umbrosum_denovo_Illumina/Illumina_assembly_PE_dedupe2/Contigs.txt f /DATOS/pgaiero/Paspalum_umbrosum_denovo_Illumina/Illumina_assembly_PE_dedupe2/Pumbrosum_subreads.fastq f /DATOS/pgaiero/Paspalum_umbrosum_denovo_Illumina/Illumina_assembly_PE_dedupe2/concatenated_MinION_pass.fq &
And here's the message I got:
Loading contigs.
Collecting information for consensus.
1317355 reads.
Calculating reads overlaps.
1000000 reads aligned.
Avg alignment size: 54
Avg sparse alignment size: 3
total alignments: 13815382
Avg alignment size: 54
Avg sparse alignment size: 3
13869759 alignments calculated.
1288 secs.
Loading non-contained sequences.
191590 loaded.
frag sum: 1072099954
offset sum: 347043943
Empty sequence loaded. It looks like you have messed up the data.
Assembly finished.
I made sure that all files were in the working directory so the problem is not the same as in this issue.
Would you mind pointing out what's wrong?
Thanks a million for your help!
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Related Issues (20)
- Empty sequence loaded. It looks like you have messed up the data. HOT 3
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- segmentation fault HOT 5
- Linked reads + PacBio reads HOT 1
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- Just give you some suggestions! HOT 1
- Floating point exception (core dumped) HOT 1
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- Smaller consensus. HOT 1
- How to use paired-end reads (R1 and R2) in SparseAssembler HOT 3
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- Error! Maximal alignment length reached. max_len: 250 HOT 2
- FreeSparseKmerGraph 出现coredump
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