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Computational pipeline for multiplexed analysis of tumors.
Hi,
We are very inspired by your work and wanted to use the code and provided analysis.
we are particularly interested in studying the dependence on single-cell protein expression however failed to find the calculation of proteinexpression_df.csv
in this repo.
In addition, trying to understand the code for protein expression calculation, calculate_protein_expression.py
, specifically in the segment assigning "positive" or negative for each protein we failed to understand the dependence on biom_expression
in line 91 as it seems like it is a variable which is updated within two internal loops in the code for general protein_expression (lines 48-50).
Thank you in advance!
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