Atomic Force Microscopy Analysis Platform.

01.03.2024 - Full Article Released in Small Methods: https://doi.org/10.1002/smtd.202301766

01.03.2024 - NanoLocz Version 1.10 Update:

• General: bug fixes, more colormaps, stacking of images/video with different pixel dimensions.
• File Openers: .asd file heights corrected.
• Simulation AFM: Parachuting option added.
• LAFM: Improved localization precision, improved auto centre for symmetrisation, height scale in nm.
• New filter: remove scars/scratches.

23.11.2023 - Preprint Released on BioRxiv - Link
Full Software version 1.0 released - Go to Releases to download the latest version
Includes several updates to file openers, stability, speed and new features.

NanoLocz is a free interactive AFM image viewer and analysis platform. It's designed for browsing and analysing height data from AFM and HS-AFM imaging with the aim of increasing throughput. see User Guide here!

Capabilities:

• Read AFM file types: .spm, .asd, .jpk, .ibw, .ARIS, .tiff, .nhf, .gwy
• Image Levelling
• Line Profiling
• Video Alignment
• Simulation AFM
• Mask Analysis
• Particle Detection (height or reference based)
• Single Particle Tracking
• Particle Alignment
• Localization AFM
• Export as: .tiff, .gif, .avi, .png, .jpeg, .pdf, .txt, .csv, .xls

Compatible file types: .spm, .asd, .jpk, .ibw, .ARIS, .tiff, .nhf, .gwy.... more being added
please request (to [email protected]) if your preferred file format is not here

Note: loading on start-up of the app can take ~1min for Mac/Windows versions
Options for Install:

1. Install as Mac desktop app download: Mac_AppInstaller_mcr.app.zip
2. Install as Windows desktop app download: Windows_AppInstaller_mcr.exe
3. Install as MATLAB app download: NanoLocz.mlappinstall Open 'NanoLocz.mlappinstall' file. This will open MATLAB if not already open and ask you to install. Once installed the app can be used from the apps tab. For quick access right click the NanoLocz app to add to Favourites and/or Quick Access Toolbar.
   MATLAB app requirements: MATLAB 2020a (the newer the better), Curve Fitting Toolbox, Image Processing Toolbox, Signal Processing Toolbox, Statistics and Machine Learning Toolbox, Bioinformatics Toolbox, Computer Vision Toolbox.

THE SOFTWARE IS PROVIDED “AS IS”, WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

Contributions are extremely welcome.

Distributed under the terms of the GNU GPL v3.0 license, "NanoLocz" is free and open source software

• If using the NanoLocz software please cite:
  Heath, G.R, Micklethwaite, E. and Storer, T.M.
  NanoLocz: Image analysis platform for AFM, high-speed AFM and localization AFM.
  Small Methods 2024, 2301766.

• If using the Localization Atomic Force Microscopy method please cite:
  Heath, G.R., Kots, E., Robertson, J.L. et al.
  Localization atomic force microscopy.
  Nature 594, 385–390 (2021)