This is a small package for extracting contextual information about dual-coding exons highlighted by Mirage2 during intra-species multiple sequence alignment.
When you first download this package, you'll need to run the pseudo-makefile
lazy_maker.sh
within the hsi
subdirectory. Then, you can use the following
to run the extractor:
./DCE-Extractor.pl [Mirage2-Results] [Species-Guide]
Where Mirage2-Results
is the output directory produced by a run of Mirage2, and
Species-Guide
is the guide file used to associate species with their genomes
during that run of Mirage2.
The results of the extractor will be placed in a directory named Extracted-DCEs/
Within this directory, there will be a CSV-formatted file for each species that
lists each collection of left- and right-hand genomic windows of 32 nucleotides
(centered around the splice-in and splice-out sites that define the dual-coding
region), organized by "groups" within gene families.
In addition to the species-specific CSV files, there is also a subdirectory for each species where the detailed protein-to-genome alignments are depicted.
Finally, there may be a file called Gappy-Sequences.out
which lists any sequences
for which the mapping of amino acids to the genomic region relevant to a dual-coding
region highlighted by Mirage2 is gappy (suggestive of low-quality alignment and less
likely to be genuinely dual-coding).