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PlantNGSTools - Plant RNA-seq analysis tool

Latest version: Latest Version

Tutorial

https://blog.ugeneyun.cn/software/PlantNGSTools.manual.html

Installation

install.packages('devtools')
devtools::install_github('biomarble/PlantNGSTools')

Module

用途 模块名 备注
DEG analysis with replicates
有生物学重复的差异表达基因分析
DEGAnalysis_DESeq2 DESeq2
DEG analysis without replicates
没有生物学重复的差异表达基因分析
DEGAnalysis_EBSeq EBSeq
Gene Ontology (GO) enrichment analysis
GO 功能富集
GOEnrich topGO
Gene Ontology (GO) enrichment analysis with eggNOG-Mapper annotation
基于eggNOG-Mapper注释的GO富集
GOEnrich_eggnog topGO
Gene Ontology (GO) enrichment analysis with PANNZER2 annotation
基于PANNZER2注释的GO富集
GOEnrich_pannzer2 topGO
Gene Ontology (GO) enrichment analysis with custom annotation
自主注释的GO富集
GOEnrich_customMapping
GOEnrich_customTable
topGO
KEGG Pathway enrichment analysis
KEGG Pathway富集
KEGGenrich -
KEGG Pathway enrichment analysis with Blastkoala annotation
基于BlastKOALA的KEGG Pathway富集
KEGGenrich_blastkoala -
KEGG Pathway enrichment analysis with custom pathway annotation
基于自主注释的KEGG Pathway富集
KEGGenrich_customTable -
DEG Volcano Plot
差异表达分析火山图
VolcanoPlot ggplot2
DEG MA Plot
差异表达分析MA图
MAPlot ggplot2
Bubble plot for KEGG enrichment
KEGG富集气泡图
KEGGbubble ggplot2
Bubble plot for GO enrichment
GO富集气泡图
GObubble ggplot2
Bar plot for secondary GO Terms
GO二级节点1条形图
GOBar ggplot2
Trait statistics
表型常用统计量2
multiTraitStat -
Trait plot
表型统计图3
multiTraitPlot -
Expression matrix combining
表达量矩阵按组合并
matrixGroup -

Tips

  • 任何疑问、提交Bug,请在Issues反馈

  • 扫码关注公众号,不定期发布培训课程:

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Footnotes

  1. GO的二级节点是MF、BP、CC三个主节点的直接子节点

  2. 最大/小值,均值,中位数,偏度,峰度,shannon多态性指数,变异系数

  3. 直方图,相关性散点图,相关性显著性,相关系数

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