Comments (5)
brianstock
commented on September 16, 2024
Hi, sorry about that. I don't know why that is, the plot should just adjust the source means and SDs by the TDF means and SDs. Can you can post the first few lines of the source and TDF files? Do you have multiple versions of those files?
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BELB93
commented on September 16, 2024
Hello Brian,
Thank you for getting back to me. I based my .csv files from those in the
wolves example. Here are the excerpts:
DTF file:
Meand13C SDd13C Meand15N SDd15N
Original leaves -31.3239 0 7.567272 0
Plant detritus -30.886 0 0.849082 0
Source file (I used raw data):
Source.name d13C d15N Age Category
Animal detritus -28.9291 -1.22806 7 3
Animal detritus -26.7 2.7 7 3
Thank you so much for your time.
Warm regards,
Brinna
…On Fri, 24 Jun 2022 at 16:09, Brian Stock ***@***.***> wrote:
Hi, sorry about that. I don't know why that is, the plot should just
adjust the source means and SDs by the TDF means and SDs. Can you can post
the first few lines of the source and TDF files? Do you have multiple
versions of those files?
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brianstock
commented on September 16, 2024
There's your problem - you're subtracting about 30 from the source d13C because you have -30 in the TDF file mean column.
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BELB93
commented on September 16, 2024
Ah ok, so how should I fix this? These are the mean values for the d13C.
…On Fri, 24 Jun 2022 at 18:00, Brian Stock ***@***.***> wrote:
There's your problem - you're subtracting about 30 from the source d13C
because you have -30 in the TDF file mean column.
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brianstock
commented on September 16, 2024
TDF = tissue discrimination factor, and is an offset to account for cases where the source isotope values are modified before being expressed in the mixture. This commonly happens in consumer-prey diet analyses, especially for d15N where it is often around 3 per mille (which is why it is used to estimate trophic position). For d13C, the mean TDF should be entered in the meand13C column of the TDF file, and the SD of the TDF should be entered as SDd13C.
I don't know what your analysis is for, but -30 for a d13C TDF is quite odd.
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