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fastq-join's Introduction

fastq-join

This is the work of:

Erik Aronesty (2013). TOBioiJ : "Comparison of Sequencing Utility Programs", DOI:10.2174/1875036201307010001

Changes

The Versioning here is awful. I've bumped the version with no major changes or bug fixes to the original algorithm.

Build

git clone https://github.com/brwnj/fastq-join
cd fastq-join
make

Some tests are failing when building on OS X versus Redhat 6.6. OS X gives "nan" while Redhat gives "-nan".

Example Usage

fastq-join -p 2 -m 200 test_R1.fastq test_R2.fastq -o test_%.fastq

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fastq-join's Issues

Fail when R1 reads > R2 reads

It seems the software stops to work ad exit when number of R1 reads is greater then number of R2.
The error is because in fastq-lib.cpp qual.n returns -1 for the last record of the second file you pass :
if (fq->qual.n <= 0)
return 0;
and then with other reads it consequently fails.
But when R1 is lower, we still have -1, for reads 2, but the cycle stops and the error disappear.

When R1 reads is greater then number of R2, if you exchange the file in the command line, it works again.

If you have time...
Thank you.
&

Average join length

First of all, thank you for your work. I've found the "average join length" report somewhat misleading: one may think this is the average length of joined reads. Have you considered replacing this statistic with average joined sequence length?

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