Sample_ID | Name | Index 7 | Lane |
---|---|---|---|
Truseq06 | 1_31GEX | GCAACAAA | Lane3 |
Truseq06 | 2_31GEX | TAGTTGTC | Lane3 |
Truseq06 | 3_31GEX | CGCCATCG | Lane3 |
Truseq06 | 4_31GEX | ATTGGCGT | Lane3 |
ID | reads |
---|---|
1 | V300079632_L03_1_31GEX_1.fq.gz V300079632_L03_1_31GEX_2.fq.gz |
2 | V300079632_L03_2_31GEX_1.fq.gz V300079632_L03_2_31GEX_2.fq.gz |
3 | V300079632_L03_3_31GEX_1.fq.gz V300079632_L03_3_31GEX_2.fq.gz |
4 | V300079632_L03_4_31GEX_1.fq.gz V300079632_L03_4_31GEX_2.fq.gz |
CRData
├── BC_Attached ## output of 01_batch_attachBarcodes.py
│ └── 31GEX
│ ├── 31GEX_ATTGGCGT_L003_R1.fastq.gz
│ ├── 31GEX_ATTGGCGT_L003_R2.fastq.gz
│ ├── 31GEX_CGCCATCG_L003_R1.fastq.gz
│ ├── 31GEX_CGCCATCG_L003_R2.fastq.gz
│ ├── 31GEX_GCAACAAA_L003_R1.fastq.gz
│ ├── 31GEX_GCAACAAA_L003_R2.fastq.gz
│ ├── 31GEX_TAGTTGTC_L003_R1.fastq.gz
│ └── 31GEX_TAGTTGTC_L003_R2.fastq.gz
├── BGI ## original BGI data
│ └── 31GEX
│ ├── V300079632_L03_1_31GEX_1.fq.gz
│ ├── V300079632_L03_1_31GEX_2.fq.gz
│ ├── V300079632_L03_2_31GEX_1.fq.gz
│ ├── V300079632_L03_2_31GEX_2.fq.gz
│ ├── V300079632_L03_3_31GEX_1.fq.gz
│ ├── V300079632_L03_3_31GEX_2.fq.gz
│ ├── V300079632_L03_4_31GEX_1.fq.gz
│ └── V300079632_L03_4_31GEX_2.fq.gz
├── Converted ## output of 02_run_convertHeaders.py
│ └── 31GEX ## can take this as input of cellranger
│ ├── 31GEX_S1_L003_R1_001.fastq.gz
│ ├── 31GEX_S1_L003_R2_001.fastq.gz
│ ├── 31GEX_S2_L003_R1_001.fastq.gz
│ ├── 31GEX_S2_L003_R2_001.fastq.gz
│ ├── 31GEX_S3_L003_R1_001.fastq.gz
│ ├── 31GEX_S3_L003_R2_001.fastq.gz
│ ├── 31GEX_S4_L003_R1_001.fastq.gz
│ └── 31GEX_S4_L003_R2_001.fastq.gz
-
python3 01_batch_attachBarcodes.py
to attach barcode to fastq filePlease set
BGI_prefix
(V300079632 in our case) &lst
before running the script -
python 3 02_run_convertHeaders.py
to convert to cellranger input formatPlease set dict
d
before running the script -
bash 03_homosapien_cellranger.sh
to run cellranger
Reference: https://github.com/powellgenomicslab/BGI_vs_Illumina_Benchmark https://github.com/IMB-Computational-Genomics-Lab/BGIvsIllumina_scRNASeq