francklab / dvc-simulator Goto Github PK

The following is the error that is returned immediately upon selection of a displacement in U1 with the radio button. The image stack I am using for input is 256x256x96 voxels. Is there a readily apparent user error that may be occuring? I followed the steps as delineated in the readme through step 3. I have not resized but I am unable to find a method of selecting displacement sizes. U2 and U3 displacements give similar errors as does the Update button.

Reference to non-existent field 'm'.

Error in DVC_simulator>displ1_Callback (line 1347)
m = handles.m;

Error in gui_mainfcn (line 95)
feval(varargin{:});

Error in DVC_simulator (line 54)
gui_mainfcn(gui_State, varargin{:});

Error in @(hObject,eventdata)DVC_simulator('displ1_Callback',hObject,eventdata,guidata(hObject))

Error while evaluating UIControl Callback

Hello,

I am interested in quantifying the 3D mechanical tractions of a fibroblast type cell that is completely embedded within a hydrogel. Similar to your application in your group's PNAS paper (Stout et al. vol. 113, no. 11), I am using fluorescent microspheres to track the hydrogel deformation. I was wondering if you could advise on the acceptable amount of error for this application as determined from the DVC-simulator? In addition, do you have any recommendations on reducing the error? For example, do you have a recommendation for bead sizes and perhaps the bead density (# of beads/ml) that should be used? Thanks!

Best,

Alex

Hello! What does each axis represent on the bottom half of the results screen after running "DVC_simulator?" I have some speckled images which I believe may not have adequate intensity, but am having trouble interpreting the results. I have also attached a screenshot of the results after running the simulator on one of my stacks. Thank you for your help!

Hi,
I am a little bit confused about the data format.
What do you mean the intensity values at x, y, z positions?
My data store the x,y,z coordinate of each point with time.
Is it correct?
Thanks.