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vesicle_unwrapper's Introduction

Vesicle Unwrapper

A clumsy attempt to analyse vesicle fusion data using ImageJ Macro

In an nutshell

This macro takes the vesicle membrane and stresches it flat. After that it slices the iamge to analyze the fluorescent intensity of the membrane.

Usage

Noted that so far

Membrane selection

This is an example of vesicle

Screenshot 2021-03-03 at 14 12 45

Vesicle after selctions of the membrane

Screenshot 2021-03-03 at 14 26 34

Unwrapping

VesicleUnwrapped

Background removal

maskedGUV

Runnning Average

running average

Scanning the vesicle

ScanningUnwrappedVesicle.mov
LineProfilesUnwrappedVesicle.mov

How to cite

 Thermoplasmonic induced vesicle fusion for investigating membrane protein phase affinity

 Guillermo Moreno-Pescador, Mohammad R. Arastoo, Salvatore Chiantia, Robert Daniels, Poul Martin Bendix

 bioRxiv 2022.09.19.508467; 

 doi: https://doi.org/10.1101/2022.09.19.508467

To do

  • Extend the functionality to automatically select several vesicles in the same image.
  • Automatically close unnecessary intermidiate images
  • Optimaze the code using more functions, avoiding redundant code
  • Add the intensity fits to the final plot
  • Make a more user friendly dialog box
  • Extend it for more than just 8 bit images
  • Include the Gauss line profile snipped for the anlysis of each different domain.

vesicle_unwrapper's People

Contributors

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