A clumsy attempt to analyse vesicle fusion data using ImageJ Macro
This macro takes the vesicle membrane and stresches it flat. After that it slices the iamge to analyze the fluorescent intensity of the membrane.
Noted that so far
This is an example of vesicle
Vesicle after selctions of the membrane
ScanningUnwrappedVesicle.mov
LineProfilesUnwrappedVesicle.mov
Thermoplasmonic induced vesicle fusion for investigating membrane protein phase affinity
Guillermo Moreno-Pescador, Mohammad R. Arastoo, Salvatore Chiantia, Robert Daniels, Poul Martin Bendix
bioRxiv 2022.09.19.508467;
doi: https://doi.org/10.1101/2022.09.19.508467
- Extend the functionality to automatically select several vesicles in the same image.
- Automatically close unnecessary intermidiate images
- Optimaze the code using more functions, avoiding redundant code
- Add the intensity fits to the final plot
- Make a more user friendly dialog box
- Extend it for more than just 8 bit images
- Include the Gauss line profile snipped for the anlysis of each different domain.