Comments (2)
hi @callumparr, thanks for trying out m6anet and sorry for the delay in my response
Currently the dataprep method that m6anet differs from xpore in terms of the features generated and therefore we are not able to use the eventalign.hdf5 generated from xpore. We are planning to integrate the preprocessing steps in the future so stay tune!
To answer your second question, the current release of m6anet, the probability score that it generates is not correlated with the proportion of modified reads a site has so it is not able to run differential analysis. The next release will have some quantification features and we might be able to do differential modification analysis but currently we are still validating the quantification results
For the third question, the guppy version that we use is v2.1.3 so it is rather old but in principle this should not affect the accuracy of the algorithm. This is because we are using the current intensity features as generated by nanopolish eventalign and so it does not depend on the base-calling accuracy (although this probably might affect nanopolish eventalign)
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hey @chrishendra93 thank you for the in-depth reply. It is very useful. Look forward to new releases of m6anet!
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Related Issues (20)
- Memory usage exploding HOT 3
- Empty Eventalign.txt for Negative Sense Viral RNA HOT 1
- dataprep and data.log HOT 8
- Questions about the m6A motif HOT 1
- Genome vs transcriptome alignment HOT 4
- m6anet, eventalign, galaxy HOT 3
- error with m6anet dataprep HOT 1
- yet another error with m6anet dataprep HOT 1
- running with test eventalign test file file form github produces another error HOT 8
- installation on linux failed HOT 3
- TypeError: argument should be integer or None, not 'numpy.float64' HOT 2
- Interpreting 'inference' results HOT 1
- m6anet dataprep fails to complete: cannot convert float NaN to integer HOT 3
- Issues with Read Selection HOT 2
- The position output by m6anet seems to be 1 less than the actual position HOT 1
- empty dataprep data.json and data.log HOT 5
- Arabidopsis VIRC instead of col0 (wild type) HOT 3
- probability_modified and mod_ratio are 0 HOT 2
- f5c or nanopolish HOT 3
- Disconnect between site and individual read mod probability RNA004
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