bulk RNA-seq data trimming about mixcr HOT 2 CLOSED

Hi,
It's always a good practice to run FASTQC before any analysis to gauge the initial quality of your data. Remember, most bioinformatics software operates on the principle of "garbage in, garbage out."
If your sequences contain Illumina adapter sequences, it's advisable to use Trimmomatic before executing MiXCR. This capability will be integrated into MiXCR in future versions.
While MiXCR does offer sequence trimming features, it's primarily designed for trimming primers/adapters of known length and/or sequence. Illumina adapters, on the other hand, can appear in varying lengths across reads as artifacts. The rna-seq preset in MiXCR does not perform any trimming by default.

from mixcr.

Thank you for your prompt and helpful response!

I'm really thankful for the well-crafted tools that enable me to perform the analysis I need.
I'll reanalyze the data after trimming.

Thanks!
Ji-Hye Choi

from mixcr.