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New England Biolabs Inc.'s Projects

ampseer icon ampseer

Ampseer examines reads in fastq format and identifies which multiplex PCR primer set was used to generate the SARS-CoV-2 sequencing library they are read from. It is intended to differentiate between ARTIC v3, ARTIC v4, ARTIC v4.1, VarSkip 1a, VarSkip 2, Midnight and VarSkip Long primer sets sequenced by Illumina or ONT.

bismark icon bismark

A tool to map bisulfite converted sequence reads and determine cytosine methylation states

bwa-meth icon bwa-meth

fast and accurate alignment of BS-Seq reads using bwa-mem and a 3-letter genome

checkqc icon checkqc

CheckQC inspects the content of an Illumina runfolder and determines if it passes a set of quality criteria

cruzdb icon cruzdb

python access to UCSC genomes database

domainator icon domainator

A flexible and modular software suite for domain-based gene neighborhood and protein search, extraction, and clustering.

em-seq icon em-seq

Tools and Data related to Enzymatic Methylation Sequencing

fatools icon fatools

A Python library for performing DNA fragment-analysis

flexbar icon flexbar

flexible barcode and adapter removal

formtastic icon formtastic

A Rails form builder plugin with semantically rich and accessible markup.

galaxy icon galaxy

This branch is the construction zone for building nested workflows

hisat2 icon hisat2

Graph-based alignment (Hierarchical Graph FM index)

htsjdk icon htsjdk

A Java API for high-throughput sequencing data (HTS) formats.

iwc icon iwc

Intergalactic Workflow Commission

low_bismap_methyl_calls icon low_bismap_methyl_calls

Tools to assess called methylation in low mappability regions (as defined by bismap). Aligner MapQ does not always align with low mappability

mark-nonconverted-reads icon mark-nonconverted-reads

This program examines a BAM or SAM stream for reads that contain multiple Cs in non-CpG context. It markes these reads with a tag (XX:Z:UC) and sets the vendor failed bit to prevent these reads from being used in downstream methylation calling. It also prints summary information to stderr.

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