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Comments (9)

ggabernet avatar ggabernet commented on May 28, 2024

I've checked within the enchantr 0.0.3 container and igphyml is installed in another path:

(base) [qeaga01@thanos test-raw]$ docker run -it quay.io/biocontainers/r-enchantr:0.0.3--r42hdfd78af_1
sh-5.0# /usr/local/share/igphyml/src/igphyml
sh: /usr/local/share/igphyml/src/igphyml: No such file or directory
sh-5.0# which igphyml
/usr/local/bin/igphyml
sh-5.0#

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ggabernet avatar ggabernet commented on May 28, 2024

This can be solved by changing the igphyml parameter

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toobiwankenobi avatar toobiwankenobi commented on May 28, 2024

Hi @ggabernet, thanks a lot for all the amazing work on nf-core/airrflow. I observed the same behavior with the test profile. Is there no way to fix this in the pipeline or mention it somewhere? I actually had to look for quite a while until I landed on this page.

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ggabernet avatar ggabernet commented on May 28, 2024

Hi @toobiwankenobi, glad that this pipeline can be helpful to other people too.
Which version of airrflow are you using, and which command did you use to launch the pipeline? In principle now the right path should be set by default, but let me know if it is not and I will fix it!

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toobiwankenobi avatar toobiwankenobi commented on May 28, 2024

Hi @ggabernet, see my specs below

N E X T F L O W ~ version 23.04.1
nf-core/airrflow v3.1.0-ga2a6099

nextflow run nf-core/airrflow -profile test,singularity

according to the documentation, the default is still /usr/local/share/igphyml/src/igphyml

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andreiprodan avatar andreiprodan commented on May 28, 2024

Hi @ggabernet ! I'm also testing airrflow now and ran into the same issue with running with -profile test,singularity. I checked the depot.galaxyproject.org-singularity-r-enchantr-0.1.2--r42hdfd78af_0.img image and igphyml path is /usr/local/bin/igphyml. However, running the test pipeline with "--igphyml /usr/local/bin/igphyml" does not prevent the error:

> processing file: _main.Rmd
>   |.................................                  |  64% [unnamed-chunk-3]
> Quitting from lines 136-216 [unnamed-chunk-3] (_main.Rmd)
> Error in `value[[3L]]()`:
> Backtrace:
>  1. dowser::getTrees(...)
> Warning messages:
> 1: replacing previous import 'data.table::last' by 'dplyr::last' when loading 'enchantr'
> 2: replacing previous import 'data.table::first' by 'dplyr::first' when loading 'enchantr'
> 3: replacing previous import 'data.table::between' by 'dplyr::between' when loading 'enchantr'

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andreiprodan avatar andreiprodan commented on May 28, 2024

nextflow run nf-core/airrflow -profile test,singularity --outdir output_test --skip_all_clones_report still crashes in the same process

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ssnn-airr avatar ssnn-airr commented on May 28, 2024

Hi. We are looking into this. The issue comes from the lineage tree generation, not the clone identification. If you want to skip that step, you should use --skip_lineage.

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ggabernet avatar ggabernet commented on May 28, 2024

Hi, sorry the documentation is wrong, this parameter default is updated both in the master and dev branch to match the container:

igphyml="/usr/local/bin/igphyml"

I think the error you are getting is related to something else but not the igphyml executable path. We are currently investigating this error.

I will fix the documentation regarding the default param now though!

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