Comments (9)
I've checked within the enchantr 0.0.3
container and igphyml
is installed in another path:
(base) [qeaga01@thanos test-raw]$ docker run -it quay.io/biocontainers/r-enchantr:0.0.3--r42hdfd78af_1
sh-5.0# /usr/local/share/igphyml/src/igphyml
sh: /usr/local/share/igphyml/src/igphyml: No such file or directory
sh-5.0# which igphyml
/usr/local/bin/igphyml
sh-5.0#
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This can be solved by changing the igphyml
parameter
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Hi @ggabernet, thanks a lot for all the amazing work on nf-core/airrflow
. I observed the same behavior with the test
profile. Is there no way to fix this in the pipeline or mention it somewhere? I actually had to look for quite a while until I landed on this page.
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Hi @toobiwankenobi, glad that this pipeline can be helpful to other people too.
Which version of airrflow are you using, and which command did you use to launch the pipeline? In principle now the right path should be set by default, but let me know if it is not and I will fix it!
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Hi @ggabernet, see my specs below
N E X T F L O W ~ version 23.04.1
nf-core/airrflow v3.1.0-ga2a6099
nextflow run nf-core/airrflow -profile test,singularity
according to the documentation, the default is still /usr/local/share/igphyml/src/igphyml
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Hi @ggabernet ! I'm also testing airrflow now and ran into the same issue with running with -profile test,singularity. I checked the depot.galaxyproject.org-singularity-r-enchantr-0.1.2--r42hdfd78af_0.img image and igphyml path is /usr/local/bin/igphyml. However, running the test pipeline with "--igphyml /usr/local/bin/igphyml" does not prevent the error:
> processing file: _main.Rmd
> |................................. | 64% [unnamed-chunk-3]
> Quitting from lines 136-216 [unnamed-chunk-3] (_main.Rmd)
> Error in `value[[3L]]()`:
> Backtrace:
> 1. dowser::getTrees(...)
> Warning messages:
> 1: replacing previous import 'data.table::last' by 'dplyr::last' when loading 'enchantr'
> 2: replacing previous import 'data.table::first' by 'dplyr::first' when loading 'enchantr'
> 3: replacing previous import 'data.table::between' by 'dplyr::between' when loading 'enchantr'
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nextflow run nf-core/airrflow -profile test,singularity --outdir output_test --skip_all_clones_report
still crashes in the same process
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Hi. We are looking into this. The issue comes from the lineage tree generation, not the clone identification. If you want to skip that step, you should use --skip_lineage
.
from airrflow.
Hi, sorry the documentation is wrong, this parameter default is updated both in the master and dev branch to match the container:
Line 84 in a2a6099
I think the error you are getting is related to something else but not the igphyml
executable path. We are currently investigating this error.
I will fix the documentation regarding the default param now though!
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Related Issues (20)
- Use `enchantr` validate input also for `Fastq` samplesheet HOT 1
- Fastq samplesheet auto single_cell FALSE
- Add assembled samplesheet usage docs
- Enchantr report outputs
- Check where `subject_id` is converted to `null` HOT 1
- Add info on README.md
- Add metada should add only metadata columns needed for the analyses
- Clone tables after `define clone` process only contain `IGH` - `TRB` clones
- Revert workaround for Slurm Sbatch file too large when possible HOT 1
- Add remaining R scripts to EnchantR
- Define clones with dowser HOT 1
- Make sure all immcantation references are cited (esp. new tools) HOT 1
- Test data
- Identify / remove spaces in fields in the metadata table HOT 1
- samplesheet check make character
- Report findthreshold should report chosen threshold
- IMGT/GENE-DB downloads failing HOT 2
- test data failing HOT 3
- Analyzing BCR Rep-Seq Data in nf-core/airrflow Without Primer Information HOT 2
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