Comments (3)
usage.md is updated, but:
The typical command for running the pipeline is as follows:
nextflow run nf-core/rrna-ampliseq --reads 'data' -profile standard,docker
still needs to be adjusted! Somehow havent seen that one!
edit: also table of contents needs adjustments... still imperfect
edit2: todo list for each document:
- usage.md : table of contents, "typical command", polishing, mention standard database SILVA v132 99% derep
- troubleshooting.md : complete rewrite
- output.md : add explanation for tons of output
- installation.md : seems fine
- README.md : remove link to "Reference genomes", add general short overview what pipeline does + central methods
- configuration/reference_genomes.md : delete
- configuration/adding_your_own.md : seems fine
edit3: added more todo's
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Modified:
- docs/usage.md: table of contents
- docs/README.md: remove link to docs/configuration/reference_genomes.md
- README.md: add general short overview what pipeline does + central methods
- docs/configuration/reference_genomes.md: deleted
- docs/troubleshooting.md : added meaningful guidelines to troubleshooting
Still to do:
- docs/output.md : add explanation for tons of output
edit: added changes to docs/troubleshooting.md
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Done, might need still polishing or small adjustments though.
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Related Issues (20)
- Phyloseq object creation will fail if any samples have all reads removed by the tax filtering step HOT 5
- Add blast-consensus support to Ampliseq HOT 1
- Add greengenes2 2022.10 support to Ampliseq HOT 1
- Add custom qiime reference database support to Ampliseq. HOT 2
- Edge case: Clustering with VSEARCH fails at QIIME2_INSEQ HOT 1
- Allow to analyse 454 sequencing data HOT 2
- Add option to assign ASV to multiple species with DADA2 HOT 3
- Debug information for docker-based run. HOT 4
- Allow stratified output from picrust2 HOT 4
- nf-core/ampliseq with conda - change bioconductor-biostrings HOT 2
- Launch webpage not working HOT 4
- Adding qza file for downstream analysis in R HOT 3
- When using `--vsearch_cluster`, if you have many thousands of clusters, `AMPLISEQ:FILTER_CLUSTERS` will fail with an `Argument list too long` error. HOT 8
- test_full Cannot access file fastq HOT 1
- Multipe region amplicon sequencing analysis support (5R / SMURF / q2-sidle) HOT 1
- Getting ca 50% more ASVs than when using DADA2 on QIIME2 HOT 2
- ampliseq fails during taxonomy assignation when processing ITS sequences HOT 14
- Error No subject alternative DNS name matching zenodo.org found HOT 2
- minor improvement of sort() before denoising with method = "radix HOT 2
- 12S taxonomic classification databases HOT 3
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