Comments (5)
Part of the problem remains after commit 3d96744
Qiime import is now using trimmed.* filenames which leads to sample names with prefix "trimmed.". Therefore the sample names are not matching the ID's in the metadatafile and several processes fail.
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Solved by publishing properly named symlinks for process "qiime_import" by process "trimming"
from ampliseq.
Problem: This can fail if its really using the publishDir
directive and we should use dynamic output file names instead. I'll have a look and update this accordingly. Directly using files in paths such as $PWD is not working reliably...
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I guess I'll have to find a way to rename files accordingly to metadata... or stick strictly to using the names of the metadata....
Workaround would also be to create a directory, move the trimmed files there with the same name and use these for the next step.
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Can confirm this works now :-) Its just not possible to store the same file names in the same work directory, so I create a temporary directory in our work directory for each trimming process in fec5d44 and push the trimmed files (with the same name!) into the next step :-)
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Related Issues (20)
- Add greengenes2 2022.10 support to Ampliseq HOT 1
- Add custom qiime reference database support to Ampliseq. HOT 2
- Edge case: Clustering with VSEARCH fails at QIIME2_INSEQ HOT 1
- Allow to analyse 454 sequencing data HOT 2
- Add option to assign ASV to multiple species with DADA2 HOT 3
- Debug information for docker-based run. HOT 4
- Allow stratified output from picrust2 HOT 4
- nf-core/ampliseq with conda - change bioconductor-biostrings HOT 2
- Launch webpage not working HOT 4
- Adding qza file for downstream analysis in R HOT 3
- When using `--vsearch_cluster`, if you have many thousands of clusters, `AMPLISEQ:FILTER_CLUSTERS` will fail with an `Argument list too long` error. HOT 8
- test_full Cannot access file fastq HOT 1
- Multipe region amplicon sequencing analysis support (5R / SMURF / q2-sidle) HOT 1
- Getting ca 50% more ASVs than when using DADA2 on QIIME2 HOT 2
- ampliseq fails during taxonomy assignation when processing ITS sequences HOT 14
- Error No subject alternative DNS name matching zenodo.org found HOT 2
- minor improvement of sort() before denoising with method = "radix HOT 2
- 12S taxonomic classification databases HOT 3
- Does the `gtdb` database only include Bacteria? HOT 5
- Remove PhytoRef as it's included in PR2 5.0.0 HOT 1
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