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sfchen avatar sfchen commented on May 30, 2024

gz is already supported, if you have any problem using it to parse gz file, update here.

AfterQC currently does adapter trimming automatically if it meets:
1, it is pair-end sequencing
2, the original DNA template is shorter than sequencing cycles, so that it may sequence the adapters in the read1/read2 tail.

Note that Illumina bcl2fastq already supports some adapter trimming now, so you don't need to worry about adapter any more, except the case above.

The barcode in the command line help is only for single molecule barcoding, so just ignore that.

from afterqc.

colindaven avatar colindaven commented on May 30, 2024

Ok, thanks! Seems to work well.

from afterqc.

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