Comments (2)
gz is already supported, if you have any problem using it to parse gz file, update here.
AfterQC currently does adapter trimming automatically if it meets:
1, it is pair-end sequencing
2, the original DNA template is shorter than sequencing cycles, so that it may sequence the adapters in the read1/read2 tail.
Note that Illumina bcl2fastq already supports some adapter trimming now, so you don't need to worry about adapter any more, except the case above.
The barcode
in the command line help is only for single molecule barcoding
, so just ignore that.
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Ok, thanks! Seems to work well.
from afterqc.
Related Issues (20)
- Removal of PCR/RNA primers HOT 1
- Tool to keep reads where all bases are above a specific quality score. HOT 2
- output gzipped data HOT 3
- ValueError: max() arg is an empty sequence HOT 3
- AterQc HOT 2
- Report
- output files are truncated
- filter only for poly-X but nothing else HOT 9
- Afterqc with pypy HOT 3
- Issue with overlap analysis HOT 4
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- question about AfterQC/preprocesser.py
- AfterQC total bases calculation HOT 1
- Float division by zero in circledetector.py
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- Error despite creating env with 2.7 in conda
- Remove overrepresented sequences
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- bubble
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