The CollecTRI-derived regulons contain signed transcription factor (TF) - target gene interactions compiled from 12 different resources. This collection provides an increased coverage of transcription factors and was benchmarked against other known GRNs, showing a superior performance in identifying perturbed TFs based on gene expression data using the knockTF data sets.

The CollecTRI regulons are available in the DoRothEA and decoupler packages through OmniPath. To load the CollecTRI regulons through R or python:

# processed regulons
decoupleR::get_collectri(organism='human', split_complexes=FALSE)

# raw regulons
OmnipathR::collectri(organism=9606L, genesymbols=TRUE, loops=TRUE)

# processed regulons
import decoupler as dc
dc.get_collectri(organism='human', split_complexes=False)

# raw regulons
import omnipath as op
op.interactions.CollecTRI.get(genesymbols=True, organism=9606L, loops=True)

ExTRI, HTRI, TRRUST, TFActS, IntAct, SIGNOR, CytReg, GEREDB, Pavlidis, DoRothEA A, NTNU curations

For more information about the CollecTRI-derived regulons, please check out the following scripts:

• Construction of CollecTRI regulons
• Benchmark
  • Systematic comparison
  • Evaluation of binding weights
  • Statistical evaluation
• Case study
• Manuscript figures

If you are interested in the construction of the CollecTRI meta-resource check out this repository

The CollecTRI-derived regulons are freely available to the community. The original licenses of all resources included in CollecTRI can be found here

Expanding the coverage of regulons from high-confidence prior knowledge for accurate estimation of transcription factor activities Sophia Mueller-Dott, Eirini Tsirvouli, Miguel Vazquez, Ricardo Omar Ramirez Flores, Pau Badia-i-Mompel, Robin Fallegger, Astrid Lagreid, Julio Saez-Rodriguez bioRxiv 2023.03.30.534849; doi: https://doi.org/10.1101/2023.03.30.534849