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Home Page: https://pha4ge.org/bioinformatics-pipelines-and-visualization/
License: Apache License 2.0
Bioinformatics Pipeline and Visualization resources
Home Page: https://pha4ge.org/bioinformatics-pipelines-and-visualization/
License: Apache License 2.0
The pipeline standards descriptions are fantastic, but could be improved by explicitly stating exactly how the standard can be met and how it can be verified that the standard has in fact been met.
Here I propose for each standard including 2 additional statements:
"To meet this standard..."
"To verify this standard..."
This will clear up ambiguities for both developers and reviewers. The goal is to define what pieces of information must be available to reviewers to receive verification of standards being met.
Still set as {in development}
Last updated 2021-12-07
My group and I have been reviewing the Proposed Standards for Public Health Bioinformatics Software document from the perspective of a team dedicated to the development of analysis pipelines, and we have some observations, from our humble opinion and experience, about the document, that we hope could help in its development.
We believe it needs to be clearly defined whether these are minimum requirements, best practices, or guidelines, something we think its already under discussion in the meetings. We also think it should be clarified whether these are standards for pipelines or software, as some points may not apply to pipelines, and reversal.
We also believe that, in addition to indicating how this is going to be evaluated as Frank is working in his PR (#32), it could be useful to provide another section per point with resources, such as links or documents, that can assist developers with each of the standards.
Next, I will describe our observations on some of the points:
Here is a just proposal of reorganization to reduce the list to 10, which I believe was one of the next objectives:
Many tools exist for HIV analysis. Recently, the advent of workflow managers has lead to a large number of analysis workflows comprised of numerous tools. a description of these workflows, and the internal components would be helpful for newer HIV bioinformatics scientists.
I
Potential sequencing strategies table is absent. It is currently being occupied by the subtyping tools table
We need to create an "Influenza Resources" link on the readme.
https://github.com/pha4ge/pipeline-resources/blob/main/docs/influenza-bioinfo-solutions.md
Please include MicrobeTrace (https://microbetrace.cdc.gov/MicrobeTrace/) as a bioinformatics solution for Mpox virus (mpxv) genomic analysis. Our MicrobeTrace team at CDC recommends that users load a distance matrix or distance-based edge list in CSV format. MicrobeTrace will perform better with edge lists when compared to loading large genome sequence alignments in fasta format. We're also implementing code to generate distance-based edge lists from the Nextstrain tree output.
Contact our team for a consultation or technical support at [email protected].
There exists a need to encourage burgeoning bioinformaticians to pursue the challenges presented by HIV genomics. Here, we present a guidance document designed to help public health scientists interested in performing genomic characterization, antiviral resistance detection, phylogenetic tree construction, and genomic epidemiological investigation involving HIV Next Generation Sequencing data.
There exists a need to encourage burgeoning bioinformaticians to pursue the challenges presented by HIV genomics.
We request a guidance document designed to help public health scientists interested in performing genomic characterization, antiviral resistance detection, phylogenetic tree construction, and genomic epidemiological investigation involving HIV Next Generation Sequencing data.
Please include descriptions of common open-source bioinformatics software as well as case study examples.
Resources describing:
Creation of standards by which bioinformatics resources can be systematically assessed plus a review process defining the assessment criteria and procedure
Last update made 2021-12-19
In the TB Guidance document, references are currently listed in comments. We would like to compile them into a ref section and add ref indicators in the body of the doc.
Need for PHA4GE guidance doc on AMR gene detection
Primer erosion with omicron is showing itself to be fairly important. In some cases, masking problematic amplicons may help improve the interpretability of the data. Is it worth bringing that out here? Hard to without a fairly detailed tour through the various amplicon strategies and how they're faring.
Interesting thread from Nathan Grubaugh on problems submitting B.1.621 sequences to GISAID because of a characteristic indel: https://twitter.com/NathanGrubaugh/status/1420797454782402560 - this led to GISAID adding a list of "known indels" to ease submission.
There was a suggestion to add this to the section on submitting data.
๐ Explain the Request
Reformat the doc where it is needed. The doc is a living doc so don't hesitate to propose changes.
Additional Information
If you think that some sections need review and editing please do so.
Add a guide to submit issues and pull requests as part of the document maintenance framework.
Issues can be submitted in order to request changes or additions to the documents and should be tagged with the documents they are referring to.
Pull Requests will allow for change tracking and collaborative review of any changes implemented into any document. The PRs should also be tagged with the issue they are "fixing" and they should reference the document under modification in the PR title.
This guide should be added to the README of the pipeline-solutions main page.
Issue and PR templates would be a nice addition.
In the HIV guidance document there are a number of bioinformatics tools detailed in their application to analyze HIV genomes. Each of these tools should have a reference whether it be a publication or github page.
In this issue we request that each tool be given a reference with link.
Reformat the case studies section of the HIV guidance document to be more reader-friendly. Add section breaks and titles to each case study.
Currently the case studies appear to all be part of the same section and they are not easily distinguishable by the reader.
A fork of the main PHA4GE repo including Markdown changes that reformat this section into a more readable version.
No new section need to be added, just reformatting.
We would like a table of contents added to the HIV Guidance document with links to all sections.
Currently the document is a bit difficult to navigate for a newer github user, so a table of contents would enhance accessibility.
A fork of the main PHA4GE repo including Markdown code adding a table of contents to the HIV Guidance Document.
https://github.blog/changelog/2021-04-13-table-of-contents-support-in-markdown-files/
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