Run Illumina
Demultiplex Illumina 16S rRNA data from multiple formats into a single, consistent format.
This way, downstream pipelines can make dumb assumptions about data format.
(c) 2014, Austin G. Davis-Richardson
TODO
Supported Input Types:
- FASTQ
- paired, barcoded
- paired
- QSEQ
- paired, barcoded
- paired
Example Output
Output will always be in interleaved FASTQ format with or without the barcode sequences at the end of the header.
FASTQ (paired, barcoded)
@HWUSI-EAS163FR:7:4:107:1355:1011:0:1:NNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+HWUSI-EAS163FR:7:4:107:1355:1011:0:1:NNNNNNN
BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
Barcode sequence will be at end, pairs are interleaved.
FASTQ (paired)
@HWI-6X:10323:5:101:995:948:0:1
NCTCAGCTGCCACATCCAATTATGCTCTCCAGGCCATGTGTTGGGGGCTATCTCCTTAATCAACTGACTGCTTTAATGGTAGGCAACCCCAGCACCGTCCC
+HWI-6X:10323:5:101:995:948:0:1
BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB
@HWI-6X:10323:5:101:995:948:0:2
ACTGTTTTGAAACCTCAGAAGTGAGGCAGTGTAGCATTCAAGGGTAAAGCAGCATGCTGGAGGAACGTGCGGAGAAGTTCAGGGGAGGCTTTTCTTCCTTG
+HWI-6X:10323:5:101:995:948:0:2
No sequence, pairs are interleaved.
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