Tools

DIYbiosphere

https://www.deskgen.com/landing/

https://blog.datproject.org/2017/11/28/draft-pipelines-edna-and-more/

• DNA Isolation

  • Soap
  • Ethanol
  • Lab glass
  • Pipettes
  • Small flasks (to put finished product in)
  • Cooker
  • Temperature meter
  • Gloves

• Blood type tests

  • Kit

• Hematocrit

  • Kit
  • Centrifuge

• Plant experiments

  • Flytrap
  • Mimosa
  • EKG

• EKG

  • Sound cards
  • Electrodes
  • Software

• EMG

  • Electrodes
  • Arduino
  • Software

• To look into

  • Electrophoresis
  • PCR

Existing Equipment {docsify-ignore-all}

• Microscope Levenhuk D740T trinocular Link
  • Microscope slides (~76x26x1mm) (100pcs) Link (code 5362)
  • Slides top (~22x22mm) (200pcs) Link

Nice laboratory equip shops

https://www.medihum.sk
http://www.fisherww.sk

Needed Equipment {docsify-ignore-all}

• co2 supply
• Spectrometer
• Petri dishes
  • Between 80 and 140mm
• Glasswear
  • 500ml beakers
  • Closable jars
• Chemicals
  • 70% ethanol solution, disinfectant
• Vaha
• Personal protective equipment
  • Coats
  • Glasses
  • Gloves
• Various microscopes
  *
• Visualiser
  • for glowing at visible wavelength
• Electrophoresis tank and power supply
  • 
• Centrifuge
• Agarose
• Pipettes
  • 10, 20, 50, 100ul volume
  • Adjustable pipette (1ul increments)
  • Pipette tips
• HEPA filter workbench
  • Laminar hood
  • https://wiki.london.hackspace.org.uk/view/Project:Flow_Hood
  • 
• Sterile manipulation box
• Light source (for growing)
• Thermal cycler (OpenPCR)
  • http://openpcr.org
  • https://en.wikipedia.org/wiki/Thermal_cycler
  • http://www.instructables.com/id/Arduino-PCR-thermal-cycler-for-under-85/
• Autoclave
  • Test
  • Autoclave tape
  • Microwave
  • Pressure cooker?
  • For sterilizing labware and bio material
• Incubator
  • Old egg incubator?
  • Pet heating pads with thermostat
• Refigerator freezer
  • 4C
  • -20C
  • -80C freezer
• Consumables
• Molecular biology enzymes and reagents
• Non-pathogenic ecoli bactieria
• Nitrile gloves
• liquid tubes for measuring
• Bacterial transformation buffer

Links

Biopunk manifesto

CRISPR.kitchen

DIYbio

Biohack academy - Vimeo

igem