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YoungColorectalCancer

Script to process 111 young colorectal cancer

210 retrieved

Filter by GATK4 ( NO efficient memory )

for i in *.mutect.vcf.gz
do
  nohup ./filter.sh $i >log.`basename $i .mutect.vcf.gz` &
done

Retain the passed variants

for i in *oncefiltered.vcf.gz
do
  zcat $i |awk '/^#/ || $7~/PASS/' - >`basename $i .somatic_oncefiltered.vcf.gz`.clean.vcf
done

Convert the vcf to bed

for i in *.clean.vcf
do
  vcf2bed < $i  >`basename $i .vcf`.bed
done

Combine the bed files

if [ -s merged.bed ];then rm merged.bed;fi
for i in *.clean.bed
do
  name=`basename $i .clean.bed`
  awk 'BEGIN{OFS="\t"}{print $1,$2-length($6)+2,$3,$6,$7,"'$name'"}' $i >>merged.bed
done
sort -V merged.bed >merged.sorted.bed

Annotation

annotate_variation.pl -buildver hg19 -outfile merged --otherinfo merged.sorted.bed  /database/annotation/annovar/humandb/

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