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broad-fungalgroup

Broad Fungal Genomics group scripts

This repository hosts scripts used publications to carry out fungal genomic analyses.

Genotype filtering (filterGatkGenotypes.py)

Usage example:

filterGatkGenotypes.py --min_GQ 50 --min_percent_alt_in_AD 0.8 --min_total_DP 10 ${VCF} > ${FILTERED_VCF} 2> variant_qc_genotype_filter.tsv

create whole genome fasta-alignment from VCF (SNPs only) (vcfSnpsToFasta.py)

Usage example:

python vcfSnpsToFasta.py --max_amb_samples 40 list_vcf > filtered.SNPs.fa

create gene fasta-alignment from VCF (SNPs only) (vcfSnpsToGeneFasta.py)

Usage example:

python vcfSnpsToGeneFasta.py list_of_vcf_files reference.fasta reference.gff gene_id > output.fasta

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