This code is relevant for our paper "High Coverage Highly Accurate Long-Read Sequencing of a Mouse Neuronal Cell Line Using the PacBio Revio Sequencer" where we sequenced the Neuro-2a on a Pacific Biosciences Revio sequencer. We sequenced this cell line using 3 Revio SMRT Cells with a total coverage of 98x.

If you use this code, cite our preprint at: https://doi.org/10.1101/2023.06.06.543940

Manuel JG, Heins HB, Crocker S, Neidich JA, Sadzewicz L, Tallon L, Turner TN. High Coverage Highly Accurate Long-Read Sequencing of a Mouse Neuronal Cell Line Using the PacBio Revio Sequencer. bioRxiv. 2023:2023.06.06.543940. doi: 10.1101/2023.06.06.543940.

The analyses include

• pbmm2
• deepvariant
• pbsv
• methylation
• hifiasm
• hicanu

The raw data and variant calls are available as described in our preprint at a Globus link. We also have deposited the .fastq.gz files in NCBI SRA at the following link: https://www.ncbi.nlm.nih.gov/sra/SRX23616066.