Comments (1)
hey, unfortunately PrimerMiner does not design primers automatically, just downloads the sequences and if you like generates visualizations that allow you to design the primers manually. Metabarcoding sequences are often too variable for automated primer design =)
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Related Issues (20)
- Compare each sequence against a mitochondrial reference before clustering and verify that primers are trimmed with BOLD api
- auto delete folders when downloading failed because of server error HOT 1
- add function to selectively trimm alignments HOT 1
- If no sequences are obtained, a ReadLines error occurs HOT 1
- Install issue HOT 5
- Include scoring table into primer miner it self! HOT 1
- include product annotation in mitogenome extractions! HOT 1
- 0.3 bases
- Add maximum sequence number to download!
- check how gaps are handled with ribosomal alignments
- NCBI download does currently not work HOT 2
- Error installing JAMP locally HOT 1
- Some errors when trying to batch download with 12S and 16S markers HOT 2
- Any option to get the species in each cluster? HOT 2
- update error matching
- Can't make plots from lower case fasta files
- plot_alignment step restrictions HOT 1
- not available for this version of R HOT 6
- PrimerMiner HOT 1
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