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wgmao avatar wgmao commented on August 13, 2024
  1. The input to PLIER should be a gene-by-sample matrix where rows correspond to genes. It is unlikely given the message Selecting common genes: 12549 but it is good to double check.
  2. The default option of PLIER() is to normalize the input as the first step where it z-scores every gene (row) using scale() function. If there are any genes with 0 standard deviation, you will get a lot of NA values and this will be a problem for rsvd(). If there are any NA values included in the input before scaling, this will lead to the same problem.

from plier.

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