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yechengxi avatar yechengxi commented on June 11, 2024

The command is wrong. DBG2OLC only takes reads using f, in your command you used i1 i2.

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pdimens avatar pdimens commented on June 11, 2024

@yechengxi Thanks for the clarification. There is no need to specify forward and reverse reads when calling DBG2OLC?

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pdimens avatar pdimens commented on June 11, 2024

@yechengxi after taking your advice, I have rerun the DBG2OLC, and after some time processing, a different segmentation fault/core dumped issue has emerged has emerged. Are you familiar with what may be happening here?

Loading contigs.
703999571 k-mers in round 1.
668403631 k-mers in round 2.
Analyzing reads...
File1: ../../BFT_1st_R1.fastq
File2: ../../BFT_1st_R2.fastq
Long reads indexed. 
Total Kmers: 93106107399
Matching Unique Kmers: 69090508968
Compression time: 333618 secs.
Scoring method: 3
Match method: 1
Loading long read index
Loading file: ReadsInfoFrom_BFT_1st_R1.fastq
1417904 bad reads skipped.
31147991 reads loaded.
Loading file: ReadsInfoFrom_BFT_1st_R2.fastq
3056832 bad reads skipped.
62046677 reads loaded.
1917738 selected reads.
62046677 reads loaded.
Average size: 2
Loaded.
1917738 reads.
Calculating reads overlaps, round 1
Multiple alignment for error correction.
./dbg1.sh: line 6: 13497 Segmentation fault      (core dumped) DBG2OLC LD 0 MinOverlap 31 Contigs Contigs.txt k 21 PathCovTh 2 KmerCovTh 1 f $FORWARD f $REVERSE RemoveChimera 1 ChimeraTh 2 ContigTh 2

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yechengxi avatar yechengxi commented on June 11, 2024

Please try to use one of the commands in the manual to start with.
Search for the following in the manual and you can see some examples.
Commands for Non-hybrid NGS Assembly

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pdimens avatar pdimens commented on June 11, 2024

@yechengxi that seemed to do the trick, thank you! Does there still need to be a consensus step with blasr after the DBG2OLC step, or is this the finished illumina assembly?

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yechengxi avatar yechengxi commented on June 11, 2024

Sorry for the delay. This is the finished illumina assembly.

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pdimens avatar pdimens commented on June 11, 2024

I had sorted it out, but thank you for the repeated feedback!

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