Comments (7)
The command is wrong. DBG2OLC only takes reads using f, in your command you used i1 i2.
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@yechengxi Thanks for the clarification. There is no need to specify forward and reverse reads when calling DBG2OLC?
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@yechengxi after taking your advice, I have rerun the DBG2OLC, and after some time processing, a different segmentation fault/core dumped issue has emerged has emerged. Are you familiar with what may be happening here?
Loading contigs.
703999571 k-mers in round 1.
668403631 k-mers in round 2.
Analyzing reads...
File1: ../../BFT_1st_R1.fastq
File2: ../../BFT_1st_R2.fastq
Long reads indexed.
Total Kmers: 93106107399
Matching Unique Kmers: 69090508968
Compression time: 333618 secs.
Scoring method: 3
Match method: 1
Loading long read index
Loading file: ReadsInfoFrom_BFT_1st_R1.fastq
1417904 bad reads skipped.
31147991 reads loaded.
Loading file: ReadsInfoFrom_BFT_1st_R2.fastq
3056832 bad reads skipped.
62046677 reads loaded.
1917738 selected reads.
62046677 reads loaded.
Average size: 2
Loaded.
1917738 reads.
Calculating reads overlaps, round 1
Multiple alignment for error correction.
./dbg1.sh: line 6: 13497 Segmentation fault (core dumped) DBG2OLC LD 0 MinOverlap 31 Contigs Contigs.txt k 21 PathCovTh 2 KmerCovTh 1 f $FORWARD f $REVERSE RemoveChimera 1 ChimeraTh 2 ContigTh 2
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Please try to use one of the commands in the manual to start with.
Search for the following in the manual and you can see some examples.
Commands for Non-hybrid NGS Assembly
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@yechengxi that seemed to do the trick, thank you! Does there still need to be a consensus step with blasr after the DBG2OLC step, or is this the finished illumina assembly?
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Sorry for the delay. This is the finished illumina assembly.
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I had sorted it out, but thank you for the repeated feedback!
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Related Issues (20)
- Empty sequence loaded. It looks like you have messed up the data. HOT 3
- settings for 10x and 7x coverage
- segmentation fault HOT 5
- Linked reads + PacBio reads HOT 1
- Empty sequence loaded. It looks like you have messed up the data. HOT 1
- No release management? HOT 2
- Just give you some suggestions! HOT 1
- Floating point exception (core dumped) HOT 1
- merging of final backbone contigs to illumina assembly HOT 1
- Overlap and Layout Step HOT 1
- segfault HOT 1
- Smaller consensus. HOT 1
- How to use paired-end reads (R1 and R2) in SparseAssembler HOT 3
- 0 reads loaded -> floating point exception HOT 3
- Sparc always uses 64 CPU threads
- Error! Maximal alignment length reached. max_len: 250 HOT 2
- FreeSparseKmerGraph 出现coredump
- Is it ContigTh or ContigCovTh? HOT 1
- can dbg2olc restarts at where it fails
- Empty sequence loaded. It looks like you have messed up the data. Assembly finished. HOT 1
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