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bcb420-geo-gene's Introduction

BCB420_GEO_GENE

Usage

The usage is explained in ./R/main.R. The main step is to create a JSON file as described below, and edit the parameters at the top of the R script.

Parameters to edit:

# json filename
inputFile <- "sample.json"

# output filename for RData
outputFile <- "sampletable.RData"

# log file
logFile <- "sample.log"

JSON Format

Each experiment (comparison) is considered to be a JSON object, with the parameters "geoSeries", "description", "controlSamples", and "experimentalSamples". The values for each parameter should be a string. "geoSeries" describes the GEO Series identifier. "description" should be a unique description or descriptor for the experiment. "controlSamples" defines the normal sample(s), and "experimentalSamples" defines the experimental sample(s).

The JSON file is an array of experiments.

Sample JSON input:

[{
		"geoSeries": "GSE35330",
		"description": "Experiment 1",
		"controlSamples": ["GSM866121", "GSM866122", "GSM866124", "GSM866126"],
		"experimentalSamples": ["GSM866111", "GSM866116", "GSM866118", "GSM866119"]
	}
]

References

Utilized Boris Steipe's GEO expression analysis code: https://github.com/hyginn/zu

https://github.com/hyginn/zu/blob/master/inst/scripts/expressionAnalysisSampleCode.R

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